CHARACTERIZATION AND PERFORMANCE OF A NEUTRAL HYDROPHILIC COATING FOR THE CAPILLARY ELECTROPHORETIC SEPARATION OF BIOPOLYMERS
CHARACTERIZATION AND PERFORMANCE OF A NEUTRAL HYDROPHILIC COATING FOR THE CAPILLARY ELECTROPHORETIC SEPARATION OF BIOPOLYMERS
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DOI：
10.1016/0021-9673(93)80655-r
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发表时间：
1993-10-15
影响因子：
4.1
通讯作者：
KARGER, BL
中科院分区：
文献类型：
--
作者：
SCHMALZING, D;PIGGEE, CA;KARGER, BL
合成了聚乙烯基甲基丁二醇（50%乙烯基），并与交联剂组合用于静态涂布到熔融二氧化硅柱上。在交联和结合到表面后，将线性聚丙烯酰胺接枝到丁二醇的双键上;随后，将该线性聚合物基质与甲醛交联。接枝的中性聚合物层提供电渗流的抑制和最小化的吸附。这种组合产生了成功的蛋白质，肽和DNA分子的开管和聚合物网络分离。非常高的效率（CA。1 . 10（6）板/m），并进行了数百次连续运行，迁移时间变化极小。
Polyvinylmethylsiloxanediol (50% vinyl) was synthesized and combined with a cross-linker for static coating onto fused-silica columns. After cross-linking and binding to the surface, linear polyacrylamide was grafted to the double bonds of the siloxanediol; subsequently, this linear polymer matrix was cross-linked with formaldehyde. The grafted neutral polymeric layer provided suppression of electroosmotic flow and minimized adsorption. This combination yielded successful open tube and polymer network separations of proteins, peptides and DNA molecules. Very high efficiencies (ca. 1 . 10(6) plates/m) were achieved for open tube protein separations, and hundreds of consecutive runs were performed with minimal change in migration times.