Complete genome sequence of an Iranian isolate of Potato virus X from the legume plant Pisum sativum
Complete genome sequence of an Iranian isolate of Potato virus X from the legume plant Pisum sativum
复制标题
DOI：
10.1007/s11262-009-0371-0
复制
发表时间：
2009-05
期刊：
影响因子：
1.6
通讯作者：
N. Esfandiari;M. Kohi-Habibi;T. Hohn;M. Pooggin
中科院分区：
文献类型：
--
作者：
N. Esfandiari;M. Kohi-Habibi;T. Hohn;M. Pooggin
马铃薯病毒X （PVX）的天然寄主范围迄今仅限于苋科、十字花科和茄科。在这里，我们提供的证据表明，在伊朗PVX也发生在豌豆（Pisum sativum），一种豆科植物。为了了解病毒宿主范围扩大的分子基础，我们对PVX伊朗分离株（PVX- iran）的完整RNA基因组进行了测序，并将其与14个已知PVX分离株的基因组进行了比较。PVX-Iran核苷酸序列与欧亚分离株的同源性为99.4 - 96.7%，与南美分离株的同源性为77.2-78.0%，与所有其他分离株一样，编码rna依赖性rna聚合酶（RdRP）、三基因阻断蛋白（TGBp） 1、2、3和外壳蛋白（CP）。在氨基酸水平上，PVX-Iran与韩国分离株（PVX-KR）亲缘关系最密切，同源性为98.1% ~ 99.2%，相似度为98.6% ~ 100%。进一步检测发现，PVX- iran中有几个独特的氨基酸取代，在其他分离物中没有发生，最明显的是RdRP中的A542， TGBp3中的T10和CP中的T24，这些氨基酸替换单独或联合可能有助于打破豌豆对PVX的抗性。
The natural host range of Potato virus X (PVX) has so far been restricted to the families Amaranthaceae, Cruciferae, and Solanaceae. Here we provide evidence that in Iran PVX also occurs on pea (Pisum sativum), a legume plant. To understand the molecular basis for this expansion of the virus host range, we sequenced a complete RNA genome of the Iranian isolate of PVX (PVX-Iran) and compared it with the genomes of 14 known PVX isolates. The PVX-Iran nucleotide sequence shares 95.4–96.7% identity with the Eurasian isolates and 77.2–78.0% with the South American ones and, like all other isolates, codes for RNA-dependent RNA-polymerase (RdRP), triple gene block proteins (TGBp) 1, 2, and 3, and coat protein (CP). At the amino acid level, PVX-Iran is most closely related to the Korean isolate (PVX-KR) sharing 98.1–99.2% identity and 98.6–100% similarity. Further inspection of PVX-Iran revealed several unique amino acid substitutions that do not occur in any other isolate, most notably A542 in RdRP, T10 in TGBp3, and T24 in CP, which individually or in combination could have contributed to breaking pea resistance to PVX.