In vivo time-lapse imaging of cell divisions during neurogenesis in the developing zebrafish retina
In vivo time-lapse imaging of cell divisions during neurogenesis in the developing zebrafish retina
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DOI：
10.1016/s0896-6273(03)00066-7
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发表时间：
2003-02-20
期刊：
影响因子：
16.2
通讯作者：
Harris, WA
中科院分区：
文献类型：
--
作者：
Das, T;Payer, B;Harris, WA
双光子激发显微镜用于重建活体斑马鱼胚胎视网膜的细胞分裂。与所提出的脊椎动物不对称分裂模型相反，在有丝分裂后神经元的生成过程中，斑马鱼视网膜中没有发生顶端基底细胞分裂。然而，细胞分裂方向从中央-外周到圆周的惊人转变发生在心室表面的平面内。在 sonic you (syu) 和 lakritz (lak) 突变体中，从中央-外周分裂到圆周分裂的转变不存在或延迟，这与这些突变体中神经元分化和神经发生的延迟相关。这里的重建表明，有丝分裂细胞始终通过可以不对称遗传的薄基底突与相对的基底表面保持接触。
Two-photon excitation microscopy was used to reconstruct cell divisions in living zebrafish embryonic retinas. Contrary to proposed models for vertebrate asymmetric divisions, no apico-basal cell divisions take place in the zebrafish retina during the generation of postmitotic neurons. However, a surprising shift in the orientation of cell division from central-peripheral to circumferential occurs within the plane of the ventricular surface. In the sonic you (syu) and lakritz (lak) mutants, the shift from central-peripheral to circumferential divisions is absent or delayed, correlating with the delay in neuronal differentiation and neurogenesis in these mutants. The reconstructions here show that mitotic cells always remain in contact with the opposite basal surface by means of a thin basal process that can be inherited asymmetrically.