Isotopomer analysis of production and consumption mechanisms of N2O and CH4 in an advanced wastewater treatment system.
Isotopomer analysis of production and consumption mechanisms of N2O and CH4 in an advanced wastewater treatment system.
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DOI：
10.1021/es102985u
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发表时间：
2011-02
影响因子：
11.4
通讯作者：
S. Toyoda;Yuuri Suzuki;S. Hattori;Keita Yamada;Ayako Fujii;N. Yoshida;R. Kouno;K. Murayama;
中科院分区：
文献类型：
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作者：
S. Toyoda;Yuuri Suzuki;S. Hattori;Keita Yamada;Ayako Fujii;N. Yoshida;R. Kouno;K. Murayama;
废水处理过程被认为是一氧化二氮（N（2）O）和甲烷（CH（4））的人为来源。然而，很少有研究探讨了这些温室气体在复杂的细菌系统中产生的机制和控制因素。为了阐明废水处理过程中微生物群落中N（2）O和CH（4）的产生和消耗机制，并表征人类废物来源，我们测量了它们的浓度和同位素比（元素同位素比和特定地点的NNO不对称分子的N同位素比）在东京的一个高级处理系统收集的水和气体样品。尽管该系统的N2 O和CH 4的排放量低于以往报道的典型处理系统，但生物反应池中的水被这两种气体过饱和。由同位素比值表明，主要由硝化-反硝化作用产生的N（2）O浓度在好氧池中最高（约100 mg/L）。4000%饱和度）。溶解的CH（4）浓度最高的是在流动的水（约. 3000%饱和度）。在治疗过程中逐渐下降。其碳同位素比值表明，CH（4）的减少是由细菌氧化造成的，微生物的CH（4）生产可以发生在厌氧和沉淀池。
Wastewater treatment processes are believed to be anthropogenic sources of nitrous oxide (N(2)O) and methane (CH(4)). However, few studies have examined the mechanisms and controlling factors in production of these greenhouse gases in complex bacterial systems. To elucidate production and consumption mechanisms of N(2)O and CH(4) in microbial consortia during wastewater treatment and to characterize human waste sources, we measured their concentrations and isotopomer ratios (elemental isotope ratios and site-specific N isotope ratios in asymmetric molecules of NNO) in water and gas samples collected by an advanced treatment system in Tokyo. Although the estimated emissions of N(2)O and CH(4) from the system were found to be lower than those from the typical treatment systems reported before, water in biological reaction tanks was supersaturated with both gases. The concentration of N(2)O, produced mainly by nitrifier-denitrification as indicated by isotopomer ratios, was highest in the oxic tank (ca. 4000% saturation). The dissolved CH(4) concentration was highest in in-flow water (ca. 3000% saturation). It decreased gradually during treatment. Its carbon isotope ratio indicated that the decrease resulted from bacterial CH(4) oxidation and that microbial CH(4) production can occur in anaerobic and settling tanks.