Localization of ubiquitin and ubiquitin cross-reactive protein in human and baboon endometrium and decidua during the menstrual cycle and early pregnancy
Localization of ubiquitin and ubiquitin cross-reactive protein in human and baboon endometrium and decidua during the menstrual cycle and early pregnancy
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DOI：
10.1095/biolreprod60.4.920
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发表时间：
1999-04-01
影响因子：
3.6
通讯作者：
Fleming, SD
中科院分区：
文献类型：
--
作者：
Bebington, C;Bell, SC;Fleming, SD
我们已经研究了泛素和相关的泛素交叉反应蛋白（UCRP）的分布在石蜡包埋切片的人和狒狒子宫内膜和蜕膜免疫过氧化物酶或免疫荧光细胞化学抗体提出的泛素，UCRP，CD 45和胰岛素样生长因子结合蛋白-1。抗UCRP免疫反应在非妊娠子宫内膜中表达，尤其是在腺上皮细胞中，在妊娠开始时蜕膜化的子宫内膜间质细胞中表达上调。抗UCRP免疫反应在非妊娠子宫内膜中不表达，但在妊娠期间蜕膜细胞中表达升高。免疫印迹法表明，免疫反应性主要是由于存在的泛素和UCRP结合到其他蛋白质，虽然水平的泛素蛋白结合物在怀孕期间没有实质性变化，蜕膜化是伴随着UCRP的共轭物的外观。狒狒的子宫组织表现出类似的分布的两种蛋白质，这表明狒狒可能是一个有用的模型，用于研究的作用，泛素系统和UCRP在建立怀孕的人。
We have examined the distribution of ubiquitin and the related ubiquitin cross-reactive protein (UCRP) in paraffin-embedded sections of human and baboon endometrium and decidua by immunoperoxidase or immunofluorescence cytochemistry with antibodies raised against ubiquitin, UCRP, CD45, and insulin-like growth factor-binding protein-1. Anti-ubiquitin immunoreactivity was present in the nonpregnant endometrium, particularly in the glandular epithelial cells, and up-regulated in endometrial stromal cells as they decidualized at the beginning of pregnancy, Anti-UCRP immunoreactivity was absent from nonpregnant tissue but accumulated to high levels in decidual cells during pregnancy. Western blotting indicated that immunoreactivity was primarily due to the presence of ubiquitin and UCRP conjugated to other proteins, and that although levels of ubiquitin-protein conjugates do not change substantially during pregnancy, decidualization is accompanied by the appearance of conjugates of UCRP. Baboon uterine tissues demonstrated a similar distribution of the two proteins, which indicates that the baboon may be a useful model for study of the role of the ubiquitin system and UCRP in the establishment of pregnancy in humans.