Epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage.
Epac1 protects the retina against ischemia/reperfusion-induced neuronal and vascular damage.
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DOI：
10.1371/journal.pone.0204346
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Steinle JJ
中科院分区：
文献类型：
--
作者：
Liu L;Jiang Y;Steinle JJ
We had previously reported that exchange protein for cAMP 1 (Epac1) reduced inflammatory mediators in the retina of mice and in retinal endothelial cells (REC). Since ischemia can induce retinal damage potentially through activation of inflammatory cascades, we hypothesized that Epac1 would protect the retina against neuronal and vascular damage after exposure to ischemia/reperfusion (I/R). We used Epac1 floxed and endothelial cell specific Epac1 knockout mice for this work. We exposed them to ischemia for 90 minutes followed by reperfusion. One day after I/R, some mice were used for fluorescein angiography imaging or Evan’s blue measurements of permeability. Mice were sacrificed at 2 days for neuronal measurements and at 10 days for measurements of degenerate capillaries. Data show increased leakage in the Epac1 Cre-Lox (Epac1 EC-KO) mice exposed to I/R when compared to Epac1 floxed mice with the same treatment. I/R also increased numbers of degenerate capillaries and cell loss in all retinal layers of Epac1 EC-KO mice. Retinal thickness was reduced more significantly in the Epac1 EC-KO mice compared to Epac1 floxed mice after I/R. Taken together, the data suggest that Epac1 is protective against both neuronal and vascular damage to the retina after exposure to I/R.
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影响因子：
3.7
作者：
Santos AR;Dvoriantchikova G;Li Y;Mohammad G;Abu El-Asrar AM;Wen R;Ivanov D
通讯作者：
Ivanov D
影响因子：
3.7
作者：
Liu L;Jiang Y;Steinle JJ
通讯作者：
Steinle JJ
影响因子：
9.1
作者：
Liu Jin;Yeung, Patrick Ka Kit;Chung, Sookja Kim
通讯作者：
Chung, Sookja Kim
影响因子：
4.4
作者：
Abcouwer, Steven F.;Lin, Cheng-mao;Antonetti, David A.
通讯作者：
Antonetti, David A.
影响因子：
7.7
作者：
Caballero, Sergio;Sengupta, Nilanjana;Grant, Maria B.
通讯作者：
Grant, Maria B.