EFFECT OF 5-AZACYTIDINE ON ESCHERICHIA-COLI DNA METHYLASE
EFFECT OF 5-AZACYTIDINE ON ESCHERICHIA-COLI DNA METHYLASE
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DOI：
10.1016/0006-291x(79)92154-5
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发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
FRIEDMAN, S
中科院分区：
文献类型：
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作者：
FRIEDMAN, S
5-氮杂胞苷加入到生长的E. coliK 12，导致体外检测的DNA甲基化降低。当E. coli DNA作为底物时，比使用小牛胸腺DNA时，活性的降低不是由于该药物引起的蛋白质合成抑制，因为氯霉素和利福平都不会引起酶活性的降低。这种效应对DNA（胞嘧啶-5）甲基化酶具有特异性;腺嘌呤的甲基化不受影响。抑制DNA甲基化酶50%的药物浓度与抑制细胞生长50%的药物浓度相同。
5-Azacytidine, when added to growing E. coli K12, causes a decrease in DNA methylation assayed in vitro. This decrease is greater when E. coli DNA is used as substrate than when calf thymus DNA is used. The decrease in activity is not due to the inhibition of protein synthesis caused by this drug, since neither chloramphenicol nor rifampin causes a decrease in enzyme activity. The effect is specific for the DNA(cytosine-5)methylase; the methylation of adenine is not affected. The concentration of drug that inhibits the DNA methylase by 50% is the same concentration that inhibits cell growth by 50%.