Quantum Dots Bearing Lectin-Functionalized Nanoparticles as a Platform for In Vivo Brain Imaging
Quantum Dots Bearing Lectin-Functionalized Nanoparticles as a Platform for In Vivo Brain Imaging
复制标题
带有凝集素功能化纳米颗粒的量子点作为体内脑成像的平台
DOI：
10.1021/bc8002698
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发表时间：
2008-11-01
影响因子：
4.7
通讯作者：
Jiang, Xinguo
中科院分区：
文献类型：
--
作者：
Gao, Xiaoling;Chen, Jun;Jiang, Xinguo
将成像剂递送至脑对于诊断和治疗中枢神经系统（CNS）疾病以及阐明其病理生理学是非常重要的。量子点（QDs）提供了一种具有独特物理、化学和光学性质的新型探针，成为活体分子和细胞成像的重要工具。然而，它们的稳定性差和血脑屏障渗透性低严重限制了它们在肠胃外给药后进入并作用于CNS中的靶位点的能力。在这里，我们开发了一个基于量子点的成像平台，用于脑成像，将量子点纳入聚（乙二醇）-聚（乳酸）纳米颗粒的核心，然后用麦胚凝集素功能化，并通过鼻腔应用程序传递到大脑。所得纳米颗粒具有高有效载荷能力，是水溶性的，稳定的，并显示出优异和安全的脑靶向和成像特性。由于PEG功能性末端基团可在纳米颗粒表面上获得，这种纳米探针允许各种生物配体的缀合，从而为开发用于各种CNS疾病的特异性成像剂具有相当大的潜力。
Delivery of imaging agents to the brain is highly important for the diagnosis and treatment of central nervous system (CNS) diseases, as well as the elucidation of their pathophysiology. Quantum dots (QDs) provide a novel probe with unique physical, chemical, and optical properties, and become a promising tool for in vivo molecular and cellular imaging. However, their poor stability and low blood-brain barrier permeability severely limit their ability to enter into and act on their target sites in the CNS following parenteral administration. Here, we developed a QDs-based imaging platform for brain imaging by incorporating QDs into the core of poly(ethylene glycol)-poly(lactic acid) nanoparticles, which was then functionalized with wheat germ agglutinin and delivered into the brain via nasal application. The resulting nanoparticles, with high payload capacity, are water-soluble, stable, and showed excellent and safe brain targeting and imaging properties. With PEG functional terminal groups available on the nanoparticles surface, this nanoprobe allows for conjugation of various biological ligands, holding considerable potential for the development of specific imaging agents for various CNS diseases.