HUMAN EPIDERMAL T-CELLS PREDOMINANTLY BELONG TO THE LINEAGE EXPRESSING ALPHA-BETA-T-CELL RECEPTOR
HUMAN EPIDERMAL T-CELLS PREDOMINANTLY BELONG TO THE LINEAGE EXPRESSING ALPHA-BETA-T-CELL RECEPTOR
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DOI：
10.1084/jem.171.4.997
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发表时间：
1990-04-01
影响因子：
15.3
通讯作者：
STINGL, G
中科院分区：
文献类型：
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作者：
FOSTER, CA;YOKOZEKI, H;STINGL, G
临床上表现正常的人皮肤的表皮含有表型异质的T淋巴细胞（TC）群体，其中大多数是CD 2 +/CD 3 +/CD 5+“记忆”细胞，但处于未活化状态，并表达TCR-α/。beta..与鼠皮肤相反，人表皮中只有非常少的CD 3+细胞亚群携带TCR-γ/。三角洲表皮TC主要沿基底角质形成细胞层中的网脊沿着分布，并且通常与朗格汉斯细胞（LC）紧密并置。用TC活化剂（Con A、rIL-1、rIL-2）刺激后，这些TC从表皮细胞悬液中增殖，然后评估表型特征和TCR多样性。与原位情况类似，大多数是CD 4-/CD 8 +/TCR-α/β +。另外，两种培养物含有TCR-γ/。δ +细胞;其中之一被确定为粘附的CD 4-/CD 8+群体。表皮TC在脚掌中比在检查的其他身体区域中显著（p < 0.0001）更丰富（即，40 vs. 7 CD 3+细胞/线性厘米的表皮），似乎有一个特殊的亲和力，外泌汗管的顶面表皮上皮。此外，鞋底通常含有更多数量的CD 8+相对于CD 4 + TC，而表皮CD 4/CD 8比值在躯干和四肢是相当可变的，虽然趋势也是朝着一个稍大的百分比的CD 8+细胞。总的来说，我们的数据表明，掌侧表皮有一个独特的微环境，这是负责较高密度的TC，优先CD 8+，和较低数量的LC。这项研究不仅为正常皮肤的人表皮TC群体的显著区域变异性提供了证据，而且还加强了皮肤相关淋巴组织（SALT）的概念，其中记忆TC再循环回到表皮并与驻留的抗原呈递细胞（即，LC）。
The epidermis of clinically normal-appearing human skin harbors a phenotypically heterogeneous population of T lymphocytes (TCs), the majority of which are CD2+/CD3+/CD5+ "memory" cells, but in an unactivated state, and express the TCR-.alpha./.beta.. In contrast to murine skin, only a very minor subpopulation of CD3+ cells in the human epidermis bears the TCR-.gamma./.delta.. Epidermal TCs primarily are distributed along the rete ridges in the basal keratinocyte layer and are often in close apposition to Langerhans cells (LCs). These TCs were propagated from epidermal cell suspensions after stimulation with TC activating agents (Con A, rIL-1, rIL-2), then evaluated for phenotypic features and TCR diversity. Similar to the in situ situation, most were CD4-/CD8+/TCR-a/.beta.+. In addition, two cultures contained TCR-.gamma./.delta.+cells; one of these was determined to be an adherent CD4-/CD8+ population. Epidermal TCs were significantly (p < 0.0001) more abundant in the sole than in the other body regions examined (i.e., 40 vs. 7 CD3+ cells/linear centimeter of epidermis) and seemed to have a particular affinity for the acrosyringial epithelium of eccrine sweat ducts. Moreover, the sole usually contained a greater number of CD8+ relative to CD4+ TCs, whereas the epidermal CD4/CD8 ratio in the trunk and extremities were quite variable, although the trend also was towards a slightly larger percentage of CD8+ cells. Collectively, our data suggest that the volar epidermis has a unique microenvironment which is responsible for both the higher density of TCs, preferentially CD8+, and lower number of LCs. This study has not only provided evidence for significant regional variability in the human epidermal TC population of normal skin, but also strengthens the concept for skin-associated lymphoid tissues (SALT), whereby memory TCs recirculate back to the epidermis and interact with resident antigen-presenting cells (i.e., LC).