Langerhans cells renew in the skin throughout life under steady-state conditions
Langerhans cells renew in the skin throughout life under steady-state conditions
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DOI：
10.1038/ni852
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发表时间：
2002-12-01
影响因子：
30.5
通讯作者：
Engleman, EG
中科院分区：
文献类型：
--
作者：
Merad, M;Manz, MG;Engleman, EG
Langerhans细胞（LCS）是骨髓（BM）衍生的表皮树突状细胞（DC），代表了外部环境的关键免疫障碍，但对其生命周期知之甚少。在这里，我们表明，在接受BM移植的致命照射小鼠中，宿主起源的LC至少保持18个月，而其他器官中的DC几乎在2个月内被供体细胞完全取代。在带有独立器官的寄生虫小鼠中，但共享血液循环，没有LC的混合。但是，在暴露于紫外线的皮肤中，LCS迅速消失，并在2周内被循环的LC前体取代。新LCS的募集取决于它们的CCR2趋化因子受体的表达以及发炎的皮肤的CCR2结合趋化因子的分泌。这些数据表明，在稳态条件下，LC在局部维持，但是皮肤的炎症变化会导致其血液传播LC祖细胞的替代。
Langerhans cells (LCs) are bone marrow (BM)-derived epidermal dendritic cells (DCs) that represent a critical immunologic barrier to the external environment, but little is known about their life cycle. Here, we show that in lethally irradiated mice that had received BM transplants, LCs of host origin remained for at least 18 months, whereas DCs in other organs were almost completely replaced by donor cells within 2 months. In parabiotic mice with separate organs, but a shared blood circulation, there was no mixing of LCs. However, in skin exposed to ultraviolet light, LCs rapidly disappeared and were replaced by circulating LC precursors within 2 weeks. The recruitment of new LCs was dependent on their expression of the CCR2 chemokine receptor and on the secretion of CCR2-binding chemokines by inflamed skin. These data indicate that under steady-state conditions, LCs are maintained locally, but inflammatory changes in the skin result in their replacement by blood-borne LC progenitors.