Lactobacilli and streptococci induce interleukin-12 (IL-12), IL-18, and gamma interferon production in human peripheral blood mononuclear cells
Lactobacilli and streptococci induce interleukin-12 (IL-12), IL-18, and gamma interferon production in human peripheral blood mononuclear cells
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DOI：
10.1128/iai.66.12.6058-6062.1998
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发表时间：
1998-12-01
影响因子：
3.1
通讯作者：
Julkunen, I
中科院分区：
文献类型：
--
作者：
Miettinen, M;Matikainen, S;Julkunen, I
人外周血单个核细胞（PBMC）的刺激与三个非致病性乳酸杆菌菌株和一个致病性化脓性链球菌菌株，细胞因子基因表达和蛋白质生产进行了分析。所有细菌均强烈诱导白细胞介素-1 β（IL-1 β）、IL-6和肿瘤坏死因子α mRNA表达和蛋白质产生。S.化脓性链球菌是分泌IL-12和γ干扰素（IFN-γ）的最有效的诱导剂，并且三种乳杆菌属菌株中的两种诱导IL-12和IFN-γ产生。所有菌株诱导IL-18蛋白产生。IL-10和IL-4的产生分别被弱诱导和完全不诱导。我们的数据表明，非致病性乳酸杆菌和致病性链球菌可以诱导Th 1型细胞因子IL-12，IL-18和IFN-γ在人PBMC中。
Human peripheral blood mononuclear cells (PBMC) were stimulated with three nonpathogenic Lactobacillus strains and with one pathogenic Streptococcus pyogenes strain, and cytokine gene expression and protein production were analyzed. All bacteria strongly induced interleukin-1 beta (IL-1 beta), IL-6, and tumor necrosis factor alpha mRNA expression and protein production. S. pyogenes was the most potent inducer of secretion of IL-12 and gamma interferon (IFN-gamma), and two of three Lactobacillus strains induced IL-12 and IFN-gamma production. All strains induced IL-18 protein production. IL-10 and IL-4 production was induced weakly and not at all, respectively. Our data show that nonpathogenic lactobacilli and pathogenic streptococci can induce Th1 type cytokines IL-12, IL-18, and IFN-gamma in human PBMC.