Physico/chemical characterization and preliminary human histology assessment of a β-TCP particulate material for bone augmentation
Physico/chemical characterization and preliminary human histology assessment of a β-TCP particulate material for bone augmentation
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DOI：
10.1016/j.msec.2009.04.013
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发表时间：
2009-08-31
影响因子：
7.9
通讯作者：
Marincola, Mauro
中科院分区：
文献类型：
--
作者：
Coelho, Paulo G.;Coimbra, Maria E.;Marincola, Mauro
本研究旨在对 β-TCP 颗粒接枝材料进行物理/化学表征并评估其体内性能。 SEM/TEM、EDS 和 XPS 分别用于形态和化学评估。 FTIR 用于确定 Ca-P 相特征带。进行 Rietveld 精修/XRD 光谱以进行二次相检测。粒径分布和比表面分别通过基于散射激光的技术和BET 进行评估。采用水银孔隙率测定法来确定孔径分布。为了进行体内评估，移植材料被用于 12 名患者的上颌窦提升术，并在术后 3、6 和 9 个月时进行活检。 SEM/TEM 揭示了具有互连孔的多晶粒颗粒。 EDS 显示 Ca、P 和 O，其化学计量接近理论值。 XRD/Rietveld 显示该材料呈现结晶 β-TCP，具有类似于 9% β-Ca2O7 的第二相。 FTIR 未检测到与 α-TCR 相关的条带的存在。人体组织学评估显示，3 个月时出现新形成的骨，并且骨组织程度随着体内时间的推移而增加。人体组织学表明该材料适合颌面部复杂区域的骨再生。 (C) 2009 Elsevier B.V. 保留所有权利。
This study aimed to physico/chemically characterize and evaluate the in vivo performance of a beta-TCP particulate grafting material. SEM/TEM, and EDS and XPS were used for morphology and chemistry assessment, respectively. FTIR was used to determine Ca-P phases characteristic bands. Rietveld refinement/XRD spectra was performed for secondary phase detection. Particle size distribution and specific surface were assessed by a scattering-laser based technique and BET, respectively. Mercury porosimetry was employed to determine pore-size distribution. For in vivo evaluation, the grafting material was used in 12 patients' sinus lifts, and biopsies were obtained at post-operative times of 3, 6, and 9 months. SEM/TEM revealed multigrained particles with interconnected pores. EDS showed Ca, P, and O, with stoichiometry close to theoretical values. XRD/Rietveld showed that the material presented crystalline beta-TCP with similar to 9% beta-Ca2O7 secondary phase. FTIR did not detect the presence of bands related to alpha-TCR Human histologic assessment showed that newly formed bone was present at 3 months, and degrees of bone organization increased as time elapsed in vivo. Human histology showed that the material is suitable for bone regeneration in a maxillofacial complex region. (C) 2009 Elsevier B.V. All rights reserved.