Curcumin promotes burn wound healing in mice by upregulating caveolin-1 in epidermal stem cells
Curcumin promotes burn wound healing in mice by upregulating caveolin-1 in epidermal stem cells
复制标题
姜黄素通过上调表皮干细胞中的 Caveolin-1 促进小鼠烧伤伤口愈合
DOI：
10.1002/ptr.6238
复制
发表时间：
2018
影响因子：
7.2
通讯作者：
Julin Xie
中科院分区：
文献类型：
--
作者：
Ronghua Yang;Jingru Wang;Ziheng Zhou;Shaohai Qi;Shubin Ruan;Zepeng Lin;Qi Xin;Yan Lin;Xiaodong Chen;Julin Xie
本研究旨在探讨姜黄素对表皮干细胞(ESCs)调控创面愈合的作用及其分子机制。我们用姜黄素处理小鼠皮肤组织分离的胚胎干细胞，然后用细胞计数试剂盒-8法检测表皮生长因子诱导的细胞增殖能力。通过检测胚胎干细胞中Nanog的表达来评价胚胎干细胞的多能性。此外，皮肤烧伤的小鼠接受了ESCs的治疗，其中有或没有姜黄素的预处理。然后进行组织学评估，以确定伤口评分、细胞增殖、再上皮化和伤口内毛细血管密度。姜黄素可促进人脐静脉内皮细胞的增殖，姜黄素处理的人脐静脉内皮细胞条件培养液可促进人脐静脉内皮细胞管的形成。我们还发现，姜黄素处理增加了ESCs小窝蛋白-1的表达，这是姜黄素对ESC增殖和HUVEC管形成的有利作用所必需的。接下来，使用烧伤创面愈合的小鼠模型，姜黄素治疗的ESCs表现出促进创面闭合的作用，这也需要小窝蛋白-1的表达。我们目前的研究证实了姜黄素对小鼠烧伤创面愈合的有利作用，这种作用是通过上调ESCs中的Caveolin-1来实现的，并支持姜黄素在ESC治疗皮肤创面愈合中的潜在治疗作用。
We aimed to explore the effect of curcumin on epidermal stem cells (ESCs) in regulating wound healing and the underlying molecular mechanism. We treated mouse ESCs isolated from skin tissues with curcumin, and then assessed the proliferation ability of cells induced by epidermal growth factor using cell counting kit‐8 assay. The pluripotency of ESCs was evaluated as well through examination of Nanog expression in ESCs. Further, mice with skin burns were treated with ESCs with or without curcumin pretreatments. Histological evaluations were then preformed to determine wound scores, cell proliferation, reepithelialization, and capillary density in wounds. Curcumin treatment promoted the proliferative ability of ESCs and conditioned medium from curcumin‐treated ESCs enhanced human umbilical vein endothelial cell (HUVEC) tube formation. We also found curcumin treatment elevated caveolin‐1 expression in ESCs, which was required for the beneficial effect of curcumin on ESC proliferation and HUVEC tube formation. Next, using a mouse model of burn wound healing, curcumin‐treated ESCs exhibited enhanced wound closure, which also required caveolin‐1 expression. Our current study demonstrates the beneficial effect of curcumin on burn wound healing in mice, which is mediated by upregulating caveolin‐1 in ESCs, and supports the potential therapeutic role of curcumin in ESC‐based treatment against skin wound healing.