Photochemical DNA activation
Photochemical DNA activation
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DOI：
10.1021/ol070455u
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发表时间：
2007-05-10
期刊：
影响因子：
5.2
通讯作者：
Deiters, Alexander
中科院分区：
文献类型：
--
作者：
Lusic, Hrvoje;Young, Douglas D.;Deiters, Alexander
合成了一种新的光笼化核苷，并使用标准合成条件将其掺入DNA中。这种方法能够破坏特定的氢键，并允许分析它们对DNA酶活性的贡献。用非光损伤性的UV光短暂照射导致DNA酶活性的快速decaging和几乎定量的恢复。所开发的策略有可能在寡核苷酸功能的光诱导调节中找到广泛的应用。
A new photocaged nucleoside was synthesized and incorporated into DNA with the use of standard synthesis conditions. This approach enabled the disruption of specific H-bonds and allowed for the analysis of their contribution to the activity of a DNAzyme. Brief irradiation with nonphotodamaging UV light led to rapid decaging and almost quantitative restoration of DNAzyme activity. The developed strategy has the potential to find widespread application in the light-induced regulation of oligonucleotide function.