In vivo measurement of non-keratinized squamous epithelium using a spectroscopic microendoscope with multiple source-detector separations.
In vivo measurement of non-keratinized squamous epithelium using a spectroscopic microendoscope with multiple source-detector separations.
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使用具有多个源-检测器分离的光谱显微内窥镜对非角化鳞状上皮进行体内测量。
DOI：
10.1117/12.2211866
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Muldoon,TimothyJ
中科院分区：
文献类型：
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作者：
Greening,GageJ;Rajaram,Narasimhan;Muldoon,TimothyJ
在非角化上皮中，发育异常通常出现在基底膜附近，并随着时间的推移增殖到上上皮层中。我们提出了一种非侵入性的多模式技术，结合高分辨率荧光成像和宽带亚漫反射光谱（sDRS）来监测不同组织层的健康状况。本文重点介绍了sDRS模式的特性，该模式包含374 μm和730 μm的两个源-检测器间距（SDS），因此可以用于在两种组织厚度下从人体口腔粘膜中提取体内光学参数。首先，我们提出了经验查找表（LUT）描述约化散射（μ s '）和吸收系数（μa）和绝对反射率之间的关系。LUTS提取μs'和μ a的精度分别约为4%和8%。然后，我们提出了LUT描述μ s '，μ a和采样深度之间的关系。对于374和730 μm SDS，采样深度范围分别为210-480和260-620 μm。然后，我们证明了从13名健康志愿者的内唇提取体内μ s '、μa、血红蛋白浓度、体组织氧饱和度、散射指数和采样深度的能力，以阐明在非角化鳞状上皮内从每个SDS（374和730 μm）提取的光学参数的差异。
In the non-keratinized epithelia, dysplasia typically arises near the basement membrane and proliferates into the upper epithelial layers over time. We present a non-invasive, multimodal technique combining high-resolution fluorescence imaging and broadband sub-diffuse reflectance spectroscopy (sDRS) to monitor health at various tissue layers. This manuscript focuses on characterization of the sDRS modality, which contains two source-detector separations (SDSs) of 374 μm and 730 μm, so that it can be used to extract in vivo optical parameters from human oral mucosa at two tissue thicknesses. First, we present empirical lookup tables (LUTs) describing the relationship between reduced scattering (μs') and absorption coefficients (μa) and absolute reflectance. LUTS were shown to extract μs' and μawith accuracies of approximately 4% and 8%, respectively. We then present LUTs describing the relationship between μs', μaand sampling depth. Sampling depths range between 210-480 and 260-620 μm for the 374 and 730 μm SDSs, respectively. We then demonstrate the ability to extract in vivo μs', μa, hemoglobin concentration, bulk tissue oxygen saturation, scattering exponent, and sampling depth from the inner lip of thirteen healthy volunteers to elucidate the differences in the extracted optical parameters from each SDS (374 and 730 μm) within non-keratinized squamous epithelia.