First-stage Genome-wide Gene Expression Analyses of Human Mesenchymal Stem Cells Exposed to IC50 Ni (2+) ions
First-stage Genome-wide Gene Expression Analyses of Human Mesenchymal Stem Cells Exposed to IC50 Ni (2+) ions
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DOI：
10.11223/jarde.7.107
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发表时间：
2009-12
期刊：
影响因子：
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通讯作者：
M. Taira;T. Kagiya;M. Sasaki;K. Sasaki;S. Saitoh;T. Nezu;H. Harada;S. Kimura;Y. Araki
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文献类型：
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作者：
M. Taira;T. Kagiya;M. Sasaki;K. Sasaki;S. Saitoh;T. Nezu;H. Harada;S. Kimura;Y. Araki
本研究旨在通过29 k全基因组DNA微阵列分析来评价Ni（2+）离子对培养1 d的人骨髓间充质干细胞（hMSC）的IC 50影响。结果表明，hMSC中有39个基因表达上调超过5倍，而24个基因表达下调小于0.2倍。分析表明：（1）为了保护细胞，由于细胞周期蛋白依赖性激酶抑制剂基因的上调而发生细胞周期阻滞，同时使Ni（2+）离子的细胞内转移最小化;（2）由于通道/转运蛋白相关基因的下调而引起的缺氧条件引起轻微的炎症反应，包括通过MAPK途径上调几种趋化因子配体基因，（3）细胞内微量Ni（2+）的摄入可能通过上调锌指蛋白和铁蛋白相关基因的表达而实现。调节hMSC对Ni（2+）离子反应的主系统需要在未来澄清。
The purpose of this study was to evaluate effects of IC50 Ni (2+) ions on human mesenchymal stem cells (hMSC) cultured for one day by 29k full-genome DNA microarray analyses. It became evident that 39 genes of hMSC were up-regulated more than 5-fold, while 24 genes of hMSC were down-regulated less than 0.2-fold. The analyses suggested that (1) for cell protection, cell cycle arrest due to up-regulation of cyclin-dependent kinase inhibitor genes occurred while Ni (2+) ion intracellular transfer was minimized,(2) hypoxia condition due to down-regulation of channel/transporter related genes caused minor inflammation reaction containing up-regulation of several chemokine ligand genes through MAPK pathway, and (3) intracellular intake of minimum amounts of Ni (2+) ions might take place by up-regulation of zinc finger print protein and ferritin related genes. The master system to regulate the hMSC reaction against Ni (2+) ions needs to be clarified in the future.