The yeast SRM1 protein and human RCC1 protein share analogous functions.
The yeast SRM1 protein and human RCC1 protein share analogous functions.
复制标题
酵母 SRM1 蛋白和人类 RCC1 蛋白具有相似的功能。
DOI：
10.1091/mbc.2.10.781
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
SpragueJr,GF
中科院分区：
文献类型：
--
作者：
Clark,KL;Ohtsubo,M;Nishimoto,T;Goebl,M;SpragueJr,GF
酿酒酵母蛋白 SRM1 和哺乳动物蛋白 RCC1 在整个长度上具有氨基酸序列相似性。 SRM1 是由酵母中的隐性突变定义的，它既激活交配所需的信号转导途径，又导致细胞周期停滞在 G1 期。 RCC1 的定义是仓鼠细胞中的隐性突变，该突变会导致染色体过早凝结和进入有丝分裂的其他特征。尽管这些表型暗示着看似不同的作用，但我们认为 RCC1 和 SRM1 蛋白具有相似的功能。特别是，我们发现RCC1可以补充两个独立的srm1突变的温度敏感生长表型，并且还至少部分补充与信息素反应途径激活相关的表型，例如FUS1的转录诱导。然而，RCC1 无法补充 srm1 无效等位基因。 srm1突变体表型的进一步表征揭示了质粒和染色体稳定性的缺陷，表明突变体在DNA复制、有丝分裂或其协调方面存在缺陷。最后，与 RCC1 一样，SRM1 是一种核蛋白。总之，这些数据表明 SRM1 和 RCC1 在各自的生物体中具有共同的作用。
The Saccharomyces cerevisiae protein SRM1 and the mammalian protein RCC1 have amino acid sequence similarity throughout their lengths. SRM1 was defined by a recessive mutation in yeast that both activates the signal transduction pathway required for mating and leads to arrest in the G1 phase of the cell cycle. RCC1 was defined by a recessive mutation in hamster cells that causes premature chromosome condensation and other characteristics of entry into mitosis. Despite the seemingly different roles implied by these phenotypes, we suggest that RCC1 and SRM1 proteins have similar functions. In particular, we find that RCC1 can complement the temperature-sensitive growth phenotype of two independent srm1 mutations and also complements, at least partially, phenotypes associated with activation of the pheromone response pathway, such as transcription induction of FUS1. However, RCC1 fails to complement an srm1 null allele. Further characterization of the srm1 mutant phenotype reveals a defect in plasmid and chromosome stability, suggesting that the mutants have a defect in DNA replication, mitosis, or their coordination. Finally, like RCC1, SRM1 is a nuclear protein. Together, these data imply that SRM1 and RCC1 have a common role in their respective organisms.