RELATIONSHIP BETWEEN RIBONUCLEIC ACID-SYNTHESIZING CAPACITY OF ULTRAVIOLET-IRRADIATED NEWCASTLE DISEASE VIRUS AND ITS ABILITY TO INDUCE INTERFERON
RELATIONSHIP BETWEEN RIBONUCLEIC ACID-SYNTHESIZING CAPACITY OF ULTRAVIOLET-IRRADIATED NEWCASTLE DISEASE VIRUS AND ITS ABILITY TO INDUCE INTERFERON
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DOI：
10.1128/jvi.8.4.500-508.1971
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发表时间：
1971-01-01
影响因子：
5.4
通讯作者：
BRATT, MA
中科院分区：
文献类型：
--
作者：
CLAVELL, LA;BRATT, MA
紫外辐照的新城疫病毒在体外和体内感染早期都具有诱导核糖核酸合成的能力，但已经失去了传染性，但具有诱导干扰素的能力。在大剂量紫外线照射下，rna合成能力和干扰素诱导能力同时丧失。与转录中间体相关的有限数量的碱基配对RNA参与了这种RNA合成。这些发现表明，紫外线照射病毒的单链RNA可能通过作为合成碱基配对RNA的模板来诱导干扰素。病毒的紫外线照射会分解病毒RNA，但速度太慢，不足以成为导致RNA合成能力丧失的主要原因。有证据表明，紫外线照射病毒的模板RNA被复制的较少，合成的产物比未照射病毒合成的产物小。
Ultraviolet (UV)-irradiated Newcastle disease virus which has lost its infectivity but has the capacity to induce interferon also has the capacity to induce ribonucleic acid (RNA) synthesis both in vitro and early in infection in vivo. With large doses of UV irradiation, RNA-synthesizing capacity and interferon-inducing capacity are lost in parallel. Limited amounts of base-paired RNA associated with a transcriptive intermediate are involved in this RNA synthesis. These findings suggest the possibility that the single-stranded RNA of the UV-irradiated virus induces interferon by serving as a template for the synthesis of base-paired RNA. UV irradiation of the virus breaks down viral RNA but at a rate which is too slow to be a major cause of the loss of RNA-synthesizing capacity. Evidence is presented which suggests that less of the template RNA of the UV-irradiated virus is copied and that the product which is synthesized is smaller than that synthesized by nonirradiated virus.