CmCML11 interacts with CmCAMTA5 to enhance γ-aminobutyric acid (GABA) accumulation by regulating GABA shunt in fresh-cut cantaloupe.
CmCML11 interacts with CmCAMTA5 to enhance γ-aminobutyric acid (GABA) accumulation by regulating GABA shunt in fresh-cut cantaloupe.
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DOI：
10.1016/j.plaphy.2023.108217
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发表时间：
2023-11
期刊：
影响因子：
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通讯作者：
Wanli You;Jinglin Zhang;Xueyin Ru;Feng Xu;Zhengguo Wu;P. Jin;Yong Zheng;Shifeng Cao
中科院分区：
文献类型：
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作者：
Wanli You;Jinglin Zhang;Xueyin Ru;Feng Xu;Zhengguo Wu;P. Jin;Yong Zheng;Shifeng Cao
研究了氯化钙（CaCl 2）处理对鲜切哈密瓜中γ-氨基丁酸（GABA）积累的影响及其作用机制。结果表明：1%（w/v）CaCl 2处理提高了鲜切哈密瓜中GABA含量、谷氨酸脱羧酶（GAD）和琥珀酸半醛脱氢酶（SSADH）活性，降低了谷氨酸（Glu）含量和GABA转氨酶（GABA-T）活性;在处理前6 h，处理果实中CmCML 11和CmCAMTA 5的表达量分别比对照果实提高了187.4%和165.6%。此外，CaCl 2处理还上调了GABA分流基因CmGAD 1、CmGAD 2、CmGABA-Tan和CmSSADH的表达。此外，CmCAMTA 5作为转录激活因子，可与CmGAD 1、CmGABA-T和CmSSADH启动子中的CG盒结合，激活其转录。此外，CmCML 11与CmCAMTA 5的相互作用可增强CmCAMTA 5调控的GABA分流基因的转录激活。综上所述，CaCl 2处理促进鲜切哈密瓜中GABA的积累是通过CmCML 11和CmCAMTA 5共同调节GABA分流中CmGAD 1、CmGABA-T和CmSSADH的表达而实现的。
The effect of calcium chloride (CaCl2) treatment on γ-aminobutyric acid (GABA) accumulation in fresh-cut cantaloupe and the involved mechanisms were investigated. The result showed that 1% (w/v) CaCl2treatment increased GABA content and activities of glutamate decarboxylase (GAD) and succinate semialdehyde dehydrogenase (SSADH), while decreased glutamate (Glu) content and GABA transaminase (GABA-T) activities in fresh-cut cantaloupe.CmCML11andCmCAMTA5expressions of CaCl2-treated fruit increased by 187.4% and 165.6% than control fruit in the initial 6 h. Besides, expressions of GABA shunt genes, includingCmGAD1,CmGAD2,CmGABA-TandCmSSADHwere also up-regulated by CaCl2treatment during early storage. Moreover, acting as a transcriptional activator, CmCAMTA5 could bind to the CG-box in promoters ofCmGAD1,CmGABA-TandCmSSADHand activate their transcription. Furthermore, the interaction between CmCML11 and CmCAMTA5 could enhance the transcriptional activation on GABA shunt genes which were regulated by CmCAMTA5. Collectively, our findings revealed that CaCl2treatment promoted GABA accumulation in fresh-cut cantaloupe via the combined effect of CmCML11 and CmCAMTA5 in the regulation of expressions ofCmGAD1,CmGABA-T, andCmSSADHin GABA shunt.