Laser microscopy acquisition and analysis of premotor synapses in the murine spinal cord.
Laser microscopy acquisition and analysis of premotor synapses in the murine spinal cord.
复制标题
DOI：
10.1016/j.xpro.2022.101236
复制
发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Simon CM
中科院分区：
文献类型：
--
作者：
Buettner JM;Kirmann T;Mentis GZ;Hallermann S;Simon CM
脊髓运动神经元上的突触丢失是几种神经退行性疾病的主要特征;然而，分析这些运动前突触是具有挑战性的，因为它们的尺寸小且密度高。该协议描述了共聚焦和刺激发射耗尽（STED）成像的小鼠脊髓运动前突触和他们的段特异性定量共聚焦显微镜。我们详细介绍了脊髓节段的准备，然后进行图像采集和分析。该方案能够深入分析神经变性过程中脊髓运动前突触的病理变化。有关本方案使用和执行的完整详细信息，请参阅。已识别的小鼠脊髓节段的解剖和切片脊髓运动前突触和运动神经元的荧光标记单个运动前突触的共聚焦和超分辨率采集运动神经元上运动前突触的定量脊髓运动神经元上的突触丢失是几种神经退行性疾病的主要特征;然而，分析这些运动前突触是具有挑战性的，因为它们的尺寸小且密度高。该协议描述了共聚焦和刺激发射耗尽（STED）成像的小鼠脊髓运动前突触和他们的段特异性定量共聚焦显微镜。我们详细介绍了脊髓节段的准备，然后进行图像采集和分析。该方案能够深入分析神经变性过程中脊髓运动前突触的病理变化。
Loss of synapses on spinal motor neurons is a major feature of several neurodegenerative diseases; however, analyzing these premotor synapses is challenging because of their small size and high density. This protocol describes confocal and Stimulated Emission Depletion (STED) imaging of murine spinal premotor synapses and their segment-specific quantification by confocal microscopy. We detail the preparation of spinal cord segments, followed by image acquisition and analysis. This protocol enables in-depth analysis of pathological changes in spinal premotor synapses during neurodegeneration. For complete details on the use and execution of this protocol, please refer to. Dissection and sectioning of identified murine spinal cord segments Fluorescent labeling of spinal premotor synapses and motor neurons Confocal and super-resolution acquisition of individual premotor synapses Quantification of premotor synapses onto motor neurons Loss of synapses on spinal motor neurons is a major feature of several neurodegenerative diseases; however, analyzing these premotor synapses is challenging because of their small size and high density. This protocol describes confocal and Stimulated Emission Depletion (STED) imaging of murine spinal premotor synapses and their segment-specific quantification by confocal microscopy. We detail the preparation of spinal cord segments, followed by image acquisition and analysis. This protocol enables in-depth analysis of pathological changes in spinal premotor synapses during neurodegeneration.
登录
查看更多内容
影响因子：
5.3
作者：
Rotterman, Travis M.;Nardelli, Paul;Alvarez, Francisco J.
通讯作者：
Alvarez, Francisco J.
影响因子：
3.6
作者：
Shneider, Neil A.;Brown, Meghan N.;Alvarez, Francisco J.
通讯作者：
Alvarez, Francisco J.
影响因子：
7.7
作者：
Karlocai MR;Heredi J;Benedek T;Holderith N;Lorincz A;Nusser Z
通讯作者：
Nusser Z
影响因子：
25
作者：
Boehme, Mathias A.;Beis, Christina;Sigrist, Stephan J.
通讯作者：
Sigrist, Stephan J.
影响因子：
25
作者：
Fletcher EV;Simon CM;Pagiazitis JG;Chalif JI;Vukojicic A;Drobac E;Wang X;Mentis GZ
通讯作者：
Mentis GZ