Establishment of Alb-DsRed2 transgenic rat for liver regeneration research.
Establishment of Alb-DsRed2 transgenic rat for liver regeneration research.
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用于肝再生研究的Alb-DsRed2转基因大鼠的建立。
DOI：
10.1016/j.bbrc.2003.09.230
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发表时间：
2003
影响因子：
3.1
通讯作者：
E. Kobayashi
中科院分区：
文献类型：
--
作者：
Yuki Sato;Y. Igarashi;Y. Hakamata;T. Murakami;Takashi Kaneko;Masafumi Takahashi;N. Seo;E. Kobayashi
由于血清白蛋白是由成熟肝细胞特异性产生的，因此白蛋白产生细胞的检测系统可能是可视化肝脏再生或发育的有价值的工具。我们在这里开发了一种白蛋白增强子/启动子驱动的 Alb-DsRed2 Tg 大鼠，它表达 DsRed2，具有红色荧光蛋白的肝脏特异性报告基因表达。为了研究骨髓细胞 (BMC) 向白蛋白产生细胞的转分化，将来自 Alb-DsRed2 Tg 大鼠的 BMC 注射到患有 2-乙酰氨基芴加四氯化碳引起的急性肝损伤和重复施用 CCl4 引起的慢性肝损伤的大鼠中。 BMC 注射后，受体肝脏中产生了 DsRed2 阳性细胞。慢性肝损伤模型中转分化的DsRed2阳性细胞数量较急性损伤模型有所增加。我们认为 Alb-DsRed2 Tg 大鼠非常适合研究体内肝再生。
Because serum albumin is specifically produced by mature hepatocytes, detection system of albumin producing cells could be a valuable tool to visualize liver regeneration or development. We have developed here an albumin enhancer/promoter-driven Alb-DsRed2 Tg rat that expresses DsRed2, having liver-specific reporter gene expression of red fluorescent protein. To study the transdifferentiation of bone marrow cells (BMCs) into albumin producing cells, BMCs from the Alb-DsRed2 Tg rat were injected into rats having acute liver damage caused by 2-acetylaminofluorene plus carbon tetrachloride and chronic liver damage by repeated administration of CCl4. DsRed2-positive cells were generated in the recipient liver after BMC injection. The number of transdifferentiated DsRed2-positive cells in chronic liver injury model was increased comparing with that in acute injury model. We propose that the Alb-DsRed2 Tg rat is well suited to studying in vivo liver regeneration.
影响因子：
10.5
作者：
PINKERT, CA;ORNITZ, DM;PALMITER, RD
通讯作者：
PALMITER, RD