A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes co-encapsulated metal-organic frameworks
A simple and sensitive sensor for lactose based on cascade reactions in Au nanoclusters and enzymes co-encapsulated metal-organic frameworks
复制标题
基于金纳米簇和酶共封装金属有机框架的级联反应的简单而灵敏的乳糖传感器
DOI：
10.1016/j.foodchem.2020.127863
复制
发表时间：
2021-03-01
期刊：
影响因子：
8.8
通讯作者：
Li, Xiangyang
中科院分区：
文献类型：
--
作者：
Guo, Manli;Chi, Jingtian;Li, Xiangyang
在这项工作中，获得了一种含有牛血清白蛋白稳定的金纳米簇（AuNCs）、β - 半乳糖苷酶（β - Gal）和葡萄糖氧化酶（GOx）的沸石咪唑酯骨架材料（AuNCs/β - Gal/GOx@ZIF - 8）用于检测乳糖。与其他荧光纳米材料相比，AuNCs作为ZIF - 8中的客体物种具有明显优势，特别是其极小的尺寸（<1 nm）、合成简单、生物相容性优异以及稳定性高。此外，其表面的牛血清白蛋白可促进ZIF - 8涂层的形成，因此AuNCs与酶一起被共同包封在ZIF - 8中。X射线衍射（XRD）分析表明该复合材料具有与纯ZIF - 8相似的晶体结构。荧光显微镜图像、傅里叶变换红外光谱和能量色散X射线光谱表明复合材料中存在AuNCs。由于AuNCs/β - Gal/GOx@ZIF - 8中荧光探针和猝灭剂的局部浓度较高，其猝灭速率比溶液中游离的AuNCs和酶提高了3.4倍。
In this work, one kind of zeolite imidazole frameworks containing bovine serum albumin stabilized Au nanoclusters (AuNCs), beta-galactosidase (beta-Gal) and glucose oxidase (GOx) (AuNCs/beta-Gal/GOx@ZIF-8) were obtained to detect lactose. Compared with other fluorescent nano-materials, AuNCs show distinct advantages as a guest species in ZIF-8, specifically their extremely small size ( < 1 nm), simple synthesis, excellent biocompatibility and high stability. Furthermore, the bovine serum albumin on their surfaces can promote the formation of ZIF-8 coating; thus, AuNCs were co-encapsulated in ZIF-8 with the enzymes together. X-ray diffraction (XRD) analysis indicates the composite possesses the similar crystalline structure with pure ZIF-8. Fluorescence microscope images, Fourier transform infrared spectra and energy dispersive X-ray spectroscopy indicate the presence of AuNCs in the composite. Owing to the high local concentrations of the fluorescent probe and the quenching agent in AuNCs/beta-Gal/GOx@ZIF-8, the quenching rate was enhanced 3.4-fold that of free AuNCs and enzymes in solution.