High concordance between Illumina HiSeq2500 and NextSeq500 for reduced representation bisulfite sequencing (RRBS).
High concordance between Illumina HiSeq2500 and NextSeq500 for reduced representation bisulfite sequencing (RRBS).
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DOI：
10.1016/j.gdata.2016.10.002
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发表时间：
2016-12
期刊：
影响因子：
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通讯作者：
Keniry, Andrew
中科院分区：
文献类型：
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作者：
Yin, Danqing;Ritchie, Matthew E;Jabbari, Jafar S;Beck, Tamara;Blewitt, Marnie E;Keniry, Andrew
简化代表性亚硫酸氢盐测序（RRBS）提供了一种在高CpG密度区域中以单碱基分辨率测量DNA甲基化的有效方法。该技术已在HiSeq2500上进行了广泛的测试，HiSeq2500使用4色检测方法，但尚不清楚该方法是否也适用于NextSeq500平台，NextSeq500平台采用2色检测系统。我们创建了一个RRBS文库，并在HiSeq2500和NextSeq500上对其进行测序，发现来自两台机器的读取的碱基组成没有显著差异。此外，从每种仪器的数据做出的甲基化调用是高度一致的，整个基因组的甲基化模式如预期的那样出现。因此，RRBS可以在Nextseq500上测序，其质量与HiSeq2500相当。所有测序数据以登录号GSE 87097保藏在GEO数据库中。
Reduced representation bisulfite sequencing (RRBS) provides an efficient method for measuring DNA methylation at single base resolution in regions of high CpG density. This technique has been extensively tested on the HiSeq2500, which uses a 4-colour detection method, however it is unclear if the method will also work on the NextSeq500 platform, which employs a 2-colour detection system. We created an RRBS library and sequenced it on both the HiSeq2500 and NextSeq500, and found no significant difference in the base composition of reads derived from either machine. Moreover, the methylation calls made from the data of each instrument were highly concordant, with methylation patterns across the genome appearing as expected. Therefore, RRBS can be sequenced on the Nextseq500 with comparable quality to that of the HiSeq2500. All sequencing data are deposited in the GEO database under accession number GSE87097.