Non-bilayer lipids and biological fusion intermediates
Non-bilayer lipids and biological fusion intermediates
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DOI：
10.1016/0009-3084(96)02583-2
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发表时间：
1996-07-15
影响因子：
3.4
通讯作者：
Chernomordik, L
中科院分区：
文献类型：
--
作者：
Chernomordik, L
不同的生物融合反应和纯脂质双层的融合同样受到“非双层”脂质（在水中自身不形成脂质双层的脂质）的影响。膜的脂质组成影响融合蛋白激活下游阶段和融合孔形成之前的生物融合。这些数据表明，不同融合反应中膜脂双层的实际合并是通过相同的途径进行的。非双层脂质的作用与它们在不同方向弯曲脂质单层的能力特别相关，并且似乎与膜融合的特定假设一致，表明融合通过高度弯曲的中间体——茎——融合膜的接触单层之间的局部连接——进行。
Disparate biological fusion reactions and fusion of purely lipid bilayers are similarly influenced by 'non-bilayer' lipids (lipids which do not form lipid bilayers in water by themselves). Lipid composition of membranes affects biological fusion at a stage downstream of activation of fusion proteins and prior to fusion pore formation. These data suggest that actual merger of membrane lipid bilayers in different fusion reactions proceeds via the same pathway. The effects of non-bilayer lipids specifically correlate with their ability to bend lipid monolayers in different directions, and appear to be consistent with the specific hypothesis of membrane fusion suggesting that fusion proceeds through highly bent intermediates - stalks, local connections between contacting monolayers of fusing membranes.