Toll-Like Receptor 3 Increases Allergic and Irritant Contact Dermatitis
Toll-Like Receptor 3 Increases Allergic and Irritant Contact Dermatitis
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DOI：
10.1038/jid.2014.402
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发表时间：
2015-02-01
影响因子：
6.5
通讯作者：
Katoh, Norito
中科院分区：
文献类型：
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作者：
Nakamura, Naomi;Tamagawa-Mineoka, Risa;Katoh, Norito
toll样受体3 （Toll-like receptor 3, TLR3）在非感染性炎症性疾病中的作用已得到越来越多的认识，但TLR3在炎症性皮肤病中的功能尚不清楚。我们研究了TLR3在过敏性和刺激性接触性皮炎（ICD）中的作用。toll样受体3敲除（Tlr3 KO）小鼠的接触超敏反应（CHS）低于野生型（WT）小鼠，Tlr3转基因（Tg）小鼠在2,4,6-三硝基-1-氯苯刺激后的接触超敏反应（CHS）高于野生型（WT）小鼠。Tlr3 KO小鼠免疫淋巴结细胞过继转移诱导WT受体CHS。相比之下，来自WT小鼠的过继性转移并不能完全诱导Tlr3 KO受体的CHS。施用巴豆油后，Tlr3 KO小鼠的ICD反应较低，Tlr3 Tg小鼠的ICD反应较WT小鼠大。WT小鼠和Tlr3 KO小鼠树突状细胞的成熟、迁移、抗原呈递和淋巴细胞的增殖是相似的。这些结果表明，TLR3在过敏性接触性皮炎和ICD的诱发阶段增强抗原非依赖性皮肤炎症。
There is increasing recognition of the role of Toll-like receptor 3 (TLR3) in noninfectious inflammatory diseases, but the function of TLR3 in inflammatory skin diseases is unclear. We investigated the functions of TLR3 in allergic and irritant contact dermatitis (ICD). The contact hypersensitivity (CHS) response was lower in Toll-like receptor 3 knockout (Tlr3 KO) mice, and was greater in TLR3 transgenic (Tg) mice than in wild-type (WT) mice after challenge with 2,4,6-trinitro-1-chlorobenzene. Adoptive transfer of immunized lymph node cells from Tlr3 KO mice induced CHS in WT recipients. In contrast, adoptive transfer of those from WT mice did not fully induce CHS in Tlr3 KO recipients. The ICD reaction following croton oil application was lower in Tlr3 KO mice, and was greater in TLR3 Tg mice than in WT mice. Maturation, migration, and antigen presentation of dendritic cells and proliferation of lymphocytes between WT mice and Tlr3 KO mice were comparable. These results show that TLR3 enhances antigen-independent skin inflammation in the elicitation phase of allergic contact dermatitis and in ICD.