Adhesion of human ovarian adenocarcinoma IGROV1 cells to endothelial cells is partly mediated by the αv integrins-vitronectin adhesives system and induces an alteration of endothelial integrity
Adhesion of human ovarian adenocarcinoma IGROV1 cells to endothelial cells is partly mediated by the αv integrins-vitronectin adhesives system and induces an alteration of endothelial integrity
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DOI：
10.1016/j.cellbi.2005.01.008
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发表时间：
2005-06-01
影响因子：
3.9
通讯作者：
Carreiras, F
中科院分区：
文献类型：
--
作者：
Leroy-Dudal, J;Heyman, L;Carreiras, F
一些证据表明，血管化在卵巢上皮性肿瘤的生长、局部扩张和扩散中起着重要作用。然而，卵巢癌细胞与内皮细胞的相互作用仍然知之甚少。为了研究这一过程的粘附事件，我们使用了IGROV 1人卵巢腺癌细胞系和人脐静脉内皮细胞（HUVECs）之间的体外共培养模型。IGROV 1细胞在HUVECs单层上迅速粘附。粘附被抗α v整合素和抗Vn阻断抗体抑制。而不是抗β 1整联蛋白抗体。癌细胞的锚定导致内皮完整性的破裂。如单层中孔的形成和内皮间VE-钙粘蛋白网络的消失所揭示的。考虑到卵巢癌通过血行途径传播的能力，这些体外事件可以模拟癌细胞穿过内皮屏障外渗的初步步骤。(c)2005年国际细胞生物学联合会。由爱思唯尔有限公司出版。保留所有权利。
Several lines of evidence suggest that vascularization plays an important role in the growth, local expansion and dissemination of ovarian epithelial tumours. However, the interaction of ovarian carcinoma cells with the endothelium remains poorly understood. To investigate adhesive events underlying this process, we used an in vitro model of cocultures between the IGROV1 human ovarian adenocarcinoma cell line and human umbilical vein endothelial cells (HUVECs). IGROV1 cells were shown to adhere rapidly on the HUVECs monolayer. Adhesion was inhibited by anti-alpha v integrin and anti-Vn blocking antibodies. but not by anti-beta 1 integrin antibodies. Anchorage of carcinoma cells led to the rupture of endothelial integrity. as revealed by the formation of holes in the monolayer and by the disappearance of the interendothelial VE-Cadherin network. Considering the ability of ovarian carcinoma to disseminate by a haematogenous way, these in vitro events could mimic a preliminary step for carcinoma cells crossing the endothelial barrier to extravasate. (c) 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.