Comparison of the healing of open tibial fractures covered with either muscle or fasciocutaneous tissue in a murine model
Comparison of the healing of open tibial fractures covered with either muscle or fasciocutaneous tissue in a murine model
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DOI：
10.1002/jor.20649
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发表时间：
2008-09-01
影响因子：
2.8
通讯作者：
Nanchahal, Jagdeep
中科院分区：
文献类型：
--
作者：
Harry, Lorraine E.;Sandison, Ann;Nanchahal, Jagdeep
本研究的目的是在小鼠模型中比较肌肉或筋膜皮肤组织覆盖软组织对开放性胫骨骨折愈合的影响。在小鼠中建立开放性胫骨骨折，剥离骨膜并进行髓内固定。设计实验组，以允许单独的肌肉或皮肤加筋膜直接接触愈合骨的排他性比较。为了专门评估肌肉和筋膜皮肤组织促进剥离骨膜的骨折愈合的相对有效性，将一块无菌惰性材料（聚四氟乙烯）放置在前部，不包括皮肤和筋膜（肌肉组）或后部，不包括肌肉（筋膜皮肤组）。通过组织形态计量学、定量外周计算机断层扫描（pQCT）和使用四点弯曲技术的机械测试对骨骼修复进行组织学评估和定量。这种标准化的，可重复的模型允许表征开放性骨折愈合的形态。在骨折后28天，与单独的皮肤和筋膜相比，实验性肌肉覆盖组的愈合更快。此外，与筋膜皮肤组织相比，皮质骨含量几乎高出50%，肌肉下的愈合强度是筋膜皮肤组织的三倍（单因素方差分析p < 0.05）。在我们的新型小鼠模型中，肌肉和筋膜皮肤组织的独家比较表明，肌肉在覆盖开放性胫骨骨折方面的骨折愈合速度和质量均优于上级组织。(C)2008骨科研究学会。出版社：Wiley Periodicals，Inc.
The objective of this study was to compare the effects of soft tissue coverage by either muscle or fasciocutaneous tissue on the healing of open tibial fractures in a murine model. An open tibial fracture, stripped of periosteum with intramedullary fixation, was created in mice. Experimental groups were devised to allow exclusive comparison of either muscle alone or skin plus fascia in direct contact with healing bone. To exclusively assess the relative efficacy of muscle and fasciocutaneous tissue to promote healing of a fracture stripped of periosteum, a piece of sterile inert material (polytetrafluoroethylene) was positioned anteriorly, excluding skin and fascia (muscle group) or posteriorly, excluding muscle (fasciocutaneous group). Skeletal repair was assessed histologically and quantified by histomorphometry; quantitative peripheral computed tomography (pQCT) and mechanical testing using a four-point bending technique. This standardized, reproducible model allowed characterization of the morphology of open fracture healing. At 28 days postfracture, there was faster healing in the experimental muscle coverage group compared to skin and fascia alone. Furthermore, there was almost 50% more cortical bone content and a threefold stronger union beneath muscle compared to fasciocutaneous tissue (p < 0.05 by one-way ANOVA). Exclusive comparison of muscle and fasciocutaneous tissue in our novel murine model demonstrates that muscle is superior for the coverage of open tibial fractures for both the rate and quality of fracture healing. (C) 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.