RecET-Mediated Recombineering in Acinetobacter baumannii.
RecET-Mediated Recombineering in Acinetobacter baumannii.
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RecET 介导的鲍曼不动杆菌重组工程。
DOI：
10.1007/978-1-4939-9118-1_11
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Davies,BryanW
中科院分区：
文献类型：
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作者：
Tucker,AshleyT;Powers,MatthewJ;Trent,MStephen;Davies,BryanW
鲍曼不动杆菌迅速获得抗生素耐药性，其基因组编码耐受杀菌剂和干燥的机制，增强了它在医院环境中的持久性。快速剖析西娅的工具。鲍曼基因组需要在遗传和机制水平上理解影响其弹性的细胞因素。虽然大量的临床数据已经证明了全球范围内FA的上升。鲍曼氏杆菌作为一种耐药病原体，分析其分子细节的遗传工具一直有限。本程序描述了一种用于靶向基因组编辑的重组介导型基因工程(重组工程)系统。鲍曼尼。该系统可对广泛的基因和操纵子进行定向突变，在各种菌株中具有广泛的应用前景。鲍曼尼。
Acinetobacter baumanniirapidly acquires antibiotic resistance, and its genome encodes mechanisms to tolerate biocides and desiccation, enhancing its persistence in hospital settings. Tools to rapidly dissect theA. baumanniigenome are needed to understand cellular factors that contribute to its resiliency at a genetic and mechanistic level. While a substantial amount of clinical data has documented the global rise ofA. baumanniias an antibiotic-resistant pathogen, genetic tools to dissect its molecular details have been limited. This procedure describes a recombination-mediated genetic engineering (recombineering) system for targeted genome editing ofA. baumannii. This system can perform directed mutagenesis on wide-ranging genes and operons and has broad application in various strains ofA. baumannii.