Polynucleotide melting in heavy and light water.
Polynucleotide melting in heavy and light water.
复制标题
多核苷酸在重水和轻水中熔化。
DOI：
10.1021/bi00523a016
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Blake,RD
中科院分区：
文献类型：
--
作者：
Blake,RD
R. D. Blake abstract: The effect on the melting temperature, tm, of transferring the helix-coil (h-» c) transitionfrom H20 to D20 has been examined for a wide variety of nucleic acid helixes consisting of either two or three strands twisted into either the A-or B-like secondary structure. Very high precisionin measuring the tm was achieved by monitoring the transition by the absorbance difference approximation method with as many as six different helical species mixed together in the same solution. The average standard deviation in the tm is±0.08 C and±0.11 C for the 5tm between solvents. In all cases the melting temperature in D20 is higher than in H20. The
