Prevention of postoperative adhesions by a novel honeycomb-patterned poly(lactide) film in a rat experimental model
Prevention of postoperative adhesions by a novel honeycomb-patterned poly(lactide) film in a rat experimental model
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DOI：
10.1002/jbm.b.31026
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发表时间：
2008-08-01
影响因子：
3.4
通讯作者：
Shimomura, Masatsugu
中科院分区：
文献类型：
--
作者：
Fukuhira, Yukako;Ito, Masaya;Shimomura, Masatsugu
腹腔内粘连是腹部外科的一个严重问题。本研究评价了蜂窝状聚乳酸（HCPLA）薄膜作为物理屏障预防术后粘连的性能。以二油酰磷脂酰乙醇胺（DOPE）或十二烷基丙烯酰胺和ω-羧基己基丙烯酰胺（CAP）的共聚物（HCPLA-DOPE和HCPLA-CAP）为表面活性剂制备HCPPLA膜。在体内粘连预防实验中，雄性Sprague-Dawley大鼠在中线剖腹手术前进行标准盲肠磨损。我们将2cm × 2cm的HCPLA和平膜放置在滑动界面上;未处理的大鼠形成对照组。1周后，粘连评分为0 - 4。组间评分无显著差异，但观察到粘连预防的肉眼差异。HCPLA-DOPE对去皮鸡胸肉的粘合强度（18.4 ± 1.2g）显著高于平膜的粘合强度（15.2 ± 0.8g，p < 0.05）。此外，HCPI、A-DOPE组1周后的粘附评分（1.6 +/- 0.2）显著低于对照组（3.0 +/- 0.3，p < 0.05），但与Seprafilm（TM）组（1.4 +/- 0.3）相当。这些结果证明了HCPLA-DOPE作为预防术后粘连的物理屏障的潜力。(c)2007 Wiley Periodicals，Inc.
Intraperitoneal adhesion is a serious problem concerning abdominal surgery. This study evaluated the performance of a honeycomb-patterned poly(lactide) (HCPLA) film as a physical barrier for preventing postoperative adhesion. HCPLA films were prepared using dioleoylphosphatidylethanolamine (DOPE) or a copolymer of dodecylacrylamide and omega-carboxyhexylacrylamide (CAP) as a surfactant (HCPLA-DOPE and HCPLA-CAP, respectively). In an in vivo adhesion prevention experiment, male Sprague-Dawley rats underwent standard cecum abrasion before midline laparotomy. We placed 2 cm x 2 cm HCPLA and flat films oil the gliding interfaces; untreated rats formed the control group. After 1 week, adhesion was scored from 0 to 4. No significant difference was observed in the scores among groups, but macroscopic differences in adhesion prevention were observed. The adhesive strength of HCPLA-DOPE (18.4 +/- 1.2 g) to skinless chicken breast was significantly higher than that of the flat film (15.2 +/- 0.8 g, p < 0.05). Further, the adhesion score after 1 week for the HCPI,A-DOPE group (1.6 +/- 0.2) was significantly lower than that for the control group (3.0 +/- 0.3, p < 0.05) but comparable to that for the Seprafilm (TM) group (1.4 +/- 0.3). These results demonstrated the potential of HCPLA-DOPE as a physical barrier for preventing postoperative adhesion. (c) 2007 Wiley Periodicals, Inc.