Genome-wide identification and analysis of microRNA responding to long-term waterlogging in crown roots of maize seedlings.
Genome-wide identification and analysis of microRNA responding to long-term waterlogging in crown roots of maize seedlings.
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DOI：
10.1111/j.1399-3054.2012.01653.x
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发表时间：
2013-02
影响因子：
6.4
通讯作者：
Lihong Zhai;Zhijie Liu;Xiling Zou;Yuanyuan Jiang;F. Qiu;Yong-lian Zheng;Zuxin Zhang
中科院分区：
文献类型：
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作者：
Lihong Zhai;Zhijie Liu;Xiling Zou;Yuanyuan Jiang;F. Qiu;Yong-lian Zheng;Zuxin Zhang
MicroRNAs （miRNAs）是参与植物对非生物胁迫反应的基因表达的关键转录后调节剂。然而，miRNA在玉米对涝渍的形态响应中的调控作用尚不清楚。在本研究中，我们通过RNA测序检测了两个自交系（Hz32和Mo17）玉米幼苗涝渍冠根中表达的mirna及其靶点。共发现61个成熟miRNA，包括36个已知的玉米（zma） miRNA和25个潜在的新miRNA候选者。在两个自交系中，对涝渍和对照冠根的miRNA表达量进行比较，发现32个涝渍响应miRNA，其中大多数在涝渍条件下持续下调。我们通过降解组测序确定了miRNA靶点。在降解组文库中发现了许多涉及转录调控和活性氧消除的已知miRNA靶点，并鉴定了10个新检测到的miRNA中的17个靶点。此外，还讨论了mirna介导的内涝响应和调控树冠根诱导的途径。本研究是对涝渍玉米冠根响应性mirna的全面调查。该结果将有助于了解玉米对涝渍的miRNA表达及miRNA介导的形态适应调控。
MicroRNAs (miRNAs) are critical post-transcriptional modulators of gene expression involving in plant responses to abiotic stress. However, the regulation of miRNA in the morphological response to waterlogging is poorly understood in maize. In this study, we detected miRNAs and their targets that expressed in waterlogged crown roots of maize seedlings in two inbred lines (Hz32 and Mo17) by RNA sequencing. A total of 61 mature miRNAs were found including 36 known maize (zma) miRNAs and 25 potential novel miRNA candidates. Comparison of miRNA expression in both waterlogged and control crown roots revealed 32 waterlogging-responsive miRNAs, most were consistently downregulated under waterlogging in the two inbred lines. We identified the miRNA targets through degradome sequencing. Many known miRNA targets involving in transcription regulation and reactive oxygen species elimination were found in the degradome libraries, and 17 targets of 10 newly detected miRNAs were identified as well. Moreover, the miRNA-mediated pathways that respond to waterlogging and regulate the induction of crown roots were discussed. This study is a comprehensive survey of responsive miRNAs in waterlogged maize crown roots. The results will help to understand the miRNA expression in response to waterlogging and miRNA-mediated regulation of morphological adaptation to waterlogging in maize.