Mitochondria and apicoplast of Plasmodium falciparum:: Behaviour on subcellular fractionation and the implication
Mitochondria and apicoplast of Plasmodium falciparum:: Behaviour on subcellular fractionation and the implication
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DOI：
10.1016/j.mito.2006.11.021
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发表时间：
2007-02-01
期刊：
影响因子：
4.4
通讯作者：
Kita, Kiyoshi
中科院分区：
文献类型：
--
作者：
Kobayashi, Tamaki;Sato, Shigeharu;Kita, Kiyoshi
[中英文摘要]疟疾寄生虫疟原虫线粒体和顶体。是在显微镜下观察到的，彼此非常接近。在这项研究中，我们测试了两种不同的分离技术-Percoll密度梯度离心法和荧光激活细胞器分选法-对提高从恶性疟原虫粗细胞器制备的线粒体纯度的适用性。令我们惊讶的是，在每种方法中，质外体与原质质线粒体都是不可分割的。这意味着这两个原生质细胞器是相互结合的。这是疟原虫两个细胞器之间物理结合的第一个实验证据。(C)2006年Elsevier B.V.和线粒体研究会。版权所有。
The mitochondrion and the apicoplast of the malaria parasite, Plasmodium spp. is microscopically observed in a close proximity to each other. In this study, we tested the suitability of two different separation techniques - Percoll density gradient centrifugation and fluorescence-activated organelle sorting - for improving the purity of mitochondria isolated from the crude organelle preparation of Plasmodium falciparum. To our surprise, the apicoplast was inseparable from the plasmodial mitochondrion by each method. This implies these two plasmodial organelles are bound each other. This is the first experimental evidence of a physical binding between the two organelles in Plasmodium. (c) 2006 Elsevier B.V. and Mitochondria Research Society. All rights reserved.