LOCALIZATION OF PHOSPHATIDYLINOSITOL SIGNALING COMPONENTS IN RAT TASTE CELLS - ROLE IN BITTER TASTE TRANSDUCTION
LOCALIZATION OF PHOSPHATIDYLINOSITOL SIGNALING COMPONENTS IN RAT TASTE CELLS - ROLE IN BITTER TASTE TRANSDUCTION
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DOI：
10.1073/pnas.87.19.7395
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发表时间：
1990-10-01
影响因子：
11.1
通讯作者：
SNYDER, SH
中科院分区：
文献类型：
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作者：
HWANG, PM;VERMA, A;SNYDER, SH
为了评估磷脂酰肌醇营业额在味觉传导中的作用，我们在大鼠舌中观察到45 Ca 2+、肌醇1，4，5-三磷酸受体结合位点和磷脂酰肌醇营业额的ATP依赖性内质网积累，并通过放射自显影术监测[3 H]胞苷形成的[3 H]胞苷二磷酸二酰甘油。积累的45 Ca 2+，肌醇1，4，5-三磷酸受体，和磷脂酰肌醇营业额选择性地定位于与苦味相关的轮廓乳头味蕾的顶端区域。磷脂酰肌醇周转在苦味中的作用的进一步证据是我们观察到低浓度的地那铵（一种有效的苦味促味剂）引起的肌醇1，4，5-三磷酸质量水平的快速、选择性增加。
To assess the role of phosphatidylinositol turnover in taste transduction we have visualized, in rat tongue, ATP-dependent endoplasmic reticular accumulation of 45Ca2+, inositol 1,4,5-trisphosphate receptor binding sites, and phosphatidylinositol turnover monitored by autoradiography of [3H]cytidine diphosphate diacylglycerol formed from [3H]cytidine. Accumulated 45Ca2+, inositol 1,4,5-trisphosphate receptors, and phosphatidylinositol turnover are selectively localized to apical areas of the taste buds of circumvallate papillae, which are associated with bitter taste. Further evidence for a role of phosphatidylinositol turnover in bitter taste is our observation of a rapid, selective increase in mass levels of inositil 1,4,5-triphosphate elicited by low concentrations of denatonium, a potently bitter tastant.