Profiles of PrKX expression in developmental mouse embryo and human tissues
Profiles of PrKX expression in developmental mouse embryo and human tissues
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DOI：
10.1369/jhc.4a6568.2005
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发表时间：
2005-08-01
影响因子：
3.2
通讯作者：
Kotin, RM
中科院分区：
文献类型：
--
作者：
Li, W;Yu, ZX;Kotin, RM
蛋白激酶X（PrKX）是一种I型cAMP依赖性蛋白激酶，核型上位于人类X染色体上。虽然尚未确定PrKX的具体作用，但仅通过原位杂交和北方印迹分析而非蛋白质表达来分析小鼠和人体组织中的PrKX基因表达。为了更精确地确定PrKX蛋白水平，我们开发了特异性抗PrKX抗体，并通过免疫组化检查了妊娠期小鼠胚胎切片。这些结果表明，PrKX广泛分布和高度表达在小鼠中枢神经系统和心脏组织的早期发育阶段，并在大多数器官在后期阶段，但没有检测到在结缔组织或骨。使用蛋白质印迹法检测PrKX，分析了来自八种不同成人或胎儿组织的总蛋白提取物，包括脑、心脏、肾、肝、肺、胰腺、脾和胸腺。虽然PrKX蛋白存在于每个测试的组织中，但蛋白水平根据组织类型和发育阶段而变化。在5个月大的胎儿和成人的心脏组织中发现了非常低的蛋白水平，而PrKX蛋白在胎儿脑、肾和肝组织中比相同组织类型的成人样本更丰富。
Protein kinase X (PrKX), karyotypically located on the human X chromosome, is a type I cAMP-dependent protein kinase. Although a specific role for PrKX has not yet been defined, PrKX gene expression in mouse and human tissues has been profiled only by in situ hybridization and Northern blot analyses and not by protein expression. To determine more precisely the PrKX protein levels, we developed specific anti-PrKX antibodies and examined gestationally staged mouse embryo sections by immunohistochemistry. These results showed that PrKX is ubiquitously distributed and highly expressed in murine central nervous system and heart tissues in early developmental stages and in most organs at later stages but was not detected in either connective tissues or bone. Using Western blots to detect PrKX, total protein extracts from eight different adult or fetal human tissues including brain, heart, kidney, liver, lung, pancreas, spleen, and thymus were analyzed. Although PrKX protein was present in each of the tissues tested, the protein levels varied depending on tissue type and developmental stage. Very low protein levels were found in heart tissues from a 5-month-old fetus and from an adult, whereas PrKX proteins were more abundant in fetal brain, kidney, and liver tissues compared with adult samples of the same tissue type.