Elimination of interferences in the determination of arsenic and selenium in biological samples by inductively coupled plasma mass spectrometry
Elimination of interferences in the determination of arsenic and selenium in biological samples by inductively coupled plasma mass spectrometry
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DOI：
10.1016/0003-2670(93)80251-f
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发表时间：
1993-08
影响因子：
6.2
通讯作者：
J. Goossens;F. Vanhaecke;L. Moens;R. Dams
中科院分区：
文献类型：
--
作者：
J. Goossens;F. Vanhaecke;L. Moens;R. Dams
电感耦合等离子体质谱法测定生物样品中的砷和硒时，会受到光谱干扰的影响。四极质量分析仪的分辨率不足以从ArCl+、ArAr+和So+3等多原子物种中分辨As+和Se+离子。对人血清中这一问题的研究也揭示了在有机化合物存在下对这些元素的大量非光谱干扰。结果表明，这两个问题可以很容易地克服的化学改性（添加4%的乙醇）与雾化器流量气体调节的组合。在这些条件下，使用标准加入校准允许As和Se被准确地确定在生物来源的样品。所开发的方法被应用于人血清和尿液中的砷和硒与认证值取得了良好的协议。
The determination of As and Se in biological samples by inductively coupled plasma mass spectrometry is well known to be degraded by spectral interferences. The resolution of quadrupole mass analysers is insufficient to resolve As+and Se+ions from polyatomic species such as ArCl+, ArAr+and So+3. A study of this problem in human serum also revealed substantial non-spectral interferences on these elements occuring in the presence of organic compounds. It is shown that both problems can easily be overcome by a combination of chemical modification (addition of 4% ethanol) with nebulizer flow-rate gas adjustment. Under these conditions the use of standard additions for calibration allowed As and Se to be determined accurately in samples of biological origin. The method developed was applied to human serum and urine and for both As and Se excellent agreement with certified values was obtained.