A new and safe method for stereotactically harvesting neural stem/progenitor cells from the adult rat subventricular zone
A new and safe method for stereotactically harvesting neural stem/progenitor cells from the adult rat subventricular zone
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DOI：
10.1016/j.jneumeth.2013.12.008
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发表时间：
2014-03-30
影响因子：
3
通讯作者：
Gorji, Ali
中科院分区：
文献类型：
--
作者：
Aligholi, Hadi;Hassanzadeh, Gholamreza;Gorji, Ali
背景：成体神经干/祖细胞（NS/PC）是用于中枢神经系统疾病治疗目的的杰出细胞来源之一。由于安全问题以及从活体哺乳动物脑室下区 (SVZ) 中采集这些细胞的效率问题，自体 NS/PC 移植仍然存在争议。新方法：在这种安全的新方法中，使用 16 号半自动活检针立体定向去除一块 SVZ。然后，评估获得的细胞的增殖和分化能力。此外，还采用莫里斯水迷宫、改良神经系统严重程度评分、被动回避和旷场测试来分析活检程序的安全性。结果：尽管 SVZ 标本尺寸非常小，但它可以产生大量具有分化为神经元和神经胶质细胞能力的后代。本研究中引入的活检程序对行为和神经过程没有任何影响。与现有方法比较：现有的SVZ活检方法是不可控的技术，使用注射器而不是半自动活检针通过抽吸采集脑组织。此外，以前的方法没有在行为和认知方面进行评估。结论：这项研究表明，立体定向切除成年大鼠 SVZ 以收获 NS/PC 用于自体移植具有相当大的安全性和有效性。 (C) 2014 Elsevier B.V. 保留所有权利。
Background: Adult neural stem/progenitor cells (NS/PCs) are one of the outstanding cell sources for therapeutic purposes in the central nervous system diseases. Autologous transplantation of NS/PCs still is a matter of controversy due to the safety issue as well as efficiency of harvesting these cells from the live mammalian brain subventricular zone (SVZ).New method: In this new and safe method, a 16-guage semi-automatic biopsy needle was used stereotactically to remove a piece of SVZ. Then, the proliferation and differentiation capacity of obtained cells were assessed. In addition, the safety of the biopsy procedure was analyzed employing the Morris water maze, modified neurologic severity score, passive avoidance and open field tests.Results: Despite being very small in size, the SVZ specimen could generate a large number of progeny with the ability to differentiate into neuronal and glial cells. The biopsy procedure introduced in this study did not have any impact on the behavioral and neurological processes.Comparison with existing method(s): existing SVZ biopsy methods were uncontrollable techniques which harvested brain tissue by aspiration using a syringe not a semi-automatic biopsy needle. Also, previous methods were not evaluated in terms of behavior and cognition.Conclusions: This study revealed a considerable safety and efficacy for the stereotactical removal of the adult rat SVZ to harvest NS/PCs for autologous transplantation. (C) 2014 Elsevier B.V. All rights reserved.