Plasticity of the Arabidopsis Root System under Nutrient Deficiencies
Plasticity of the Arabidopsis Root System under Nutrient Deficiencies
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DOI：
10.1104/pp.113.218453
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发表时间：
2013-09-01
期刊：
影响因子：
7.4
通讯作者：
von Wiren, Nicolaus
中科院分区：
文献类型：
--
作者：
Gruber, Benjamin D.;Giehl, Ricardo F. H.;von Wiren, Nicolaus
植物根系对不同养分缺乏的形态反应表现出特别高的变化。虽然这种变化往往决定了植物的养分效率或胁迫耐受性，但令人惊讶的是，尚未进行全面和比较分析根形态对不同养分缺乏的反应。由于其中一个原因是在琼脂培养中获得营养缺乏条件的固有困难，我们首先确定了适合在琼脂平板上产生营养缺乏植物的条件。基于仔细选择的琼脂，专门为每种营养物质被认为是，我们生长拟南芥（拟南芥）植物在四个水平的不足12种营养物质和量化的7根性状。结合生物量和元素浓度的测量，我们观察到，营养状态和类型的营养决定了根系结构（RSA）的变化的程度和类型。个别根性状的独立调节进一步指出了根组织对养分限制的不同敏感性。为了捕捉不同养分供应下RSA的变化，我们使用主成分分析，并开发了一个根可塑性图，代表在RSA的整体调制在给定的处理。这种系统的比较RSA响应养分缺乏提供了一个全面的观点，在根可塑性的整体变化引起的单一营养素的缺乏，并提供了坚实的基础，确定营养敏感的步骤，在根发育计划。
Plant roots show a particularly high variation in their morphological response to different nutrient deficiencies. Although such changes often determine the nutrient efficiency or stress tolerance of plants, it is surprising that a comprehensive and comparative analysis of root morphological responses to different nutrient deficiencies has not yet been conducted. Since one reason for this is an inherent difficulty in obtaining nutrient-deficient conditions in agar culture, we first identified conditions appropriate for producing nutrient-deficient plants on agar plates. Based on a careful selection of agar specifically for each nutrient being considered, we grew Arabidopsis (Arabidopsis thaliana) plants at four levels of deficiency for 12 nutrients and quantified seven root traits. In combination with measurements of biomass and elemental concentrations, we observed that the nutritional status and type of nutrient determined the extent and type of changes in root system architecture (RSA). The independent regulation of individual root traits further pointed to a differential sensitivity of root tissues to nutrient limitations. To capture the variation in RSA under different nutrient supplies, we used principal component analysis and developed a root plasticity chart representing the overall modulations in RSA under a given treatment. This systematic comparison of RSA responses to nutrient deficiencies provides a comprehensive view of the overall changes in root plasticity induced by the deficiency of single nutrients and provides a solid basis for the identification of nutrient-sensitive steps in the root developmental program.