Periodontal-disease-associated biofilm: A reservoir for pathogens of medical importance
Periodontal-disease-associated biofilm: A reservoir for pathogens of medical importance
复制标题
DOI：
10.1016/j.micpath.2015.09.009
复制
发表时间：
2016-05-01
影响因子：
3.8
通讯作者：
da Silva-Boghossian, Carina Maciel
中科院分区：
文献类型：
--
作者：
Vieira Colombo, Ana Paula;Magalhaes, Clarissa Bichara;da Silva-Boghossian, Carina Maciel
牙周微环境的生态多样性可能为通常不被认为是口腔微生物群成员的物种的定殖提供合适的条件。在这项调查中，我们的目的是确定具有不同牙周临床状态的个体的微生物群中与医学相关的致病物种的患病率和水平。龈下生物膜从牙周健康（H，n = 81），牙龈炎（G，n = 55），广泛侵袭性（AgP，n = 36）或慢性牙周炎（CP，n = 98）的患者，并分析了39个微生物类群使用棋盘DNA-DNA杂交技术。通过非参数和单变量相关性检验寻找组间微生物差异以及临床和微生物参数之间的关联。奈瑟氏菌属，厌氧消化链球菌、白色念珠菌、肠杆菌、铜绿假单胞菌、隐真杆菌、艰难梭菌和乌利奥森氏菌在研究人群的龈下微生物群中的平均患病率和计数较高。在患者和部位水平与牙周炎症和组织破坏更相关的菌种包括肠杆菌、C。白色念珠菌，奈瑟氏菌属，铜绿假单胞菌、O. uli、蜂房哈夫尼菌、粘质沙雷氏菌和非细菌性丝状菌（p < 0.05）。相比之下，坏死梭杆菌、嗜酸乳杆菌、金黄色葡萄球菌和肺炎链球菌与牙周健康相关（p < 0.05）。在牙周微生物群中，可以检测到高患病率和高水平的具有医学重要性的病原物种。无论它们在牙周健康或疾病中的作用如何，牙周生物膜可能是这些病原微生物传播和发展全身感染的来源。(C)2015爱思唯尔有限公司版权所有。
The ecological diversity of the periodontal microenvironment may provide suitable conditions for the colonization of species not usually considered members of the oral microbiota. In this investigation, we aimed to determine the prevalence and levels of pathogenic species of medical relevance in the microbiota of individuals with distinct periodontal clinical status. Subgingival biofilm was obtained from patients with periodontal health (H, n = 81), gingivitis (G, n = 55), generalized aggressive (AgP, n = 36) or chronic periodontitis (CP, n = 98), and analyzed for 39 microbial taxa using a checkerboard DNA-DNA hybridization technique. Microbial differences among groups, as well as associations between clinical and microbiological parameters were sought by non-parametric and univariate correlation tests. Neisseria spp., Peptostreptococus anaerobius, Candida albicans, enterobacteria, Pseudomonas aeruginosa, Eubacterium saphenum, Clostridium difficile and Olsenella uli were detected in high mean prevalence and counts in the subgingival microbiota of the study population. Species that were more related to periodontal inflammation and tissue destruction at the patient and site levels included enterobacteria, C. albicans, Neisseria spp., P. aeruginosa, O. uli, Hafnia alvei, Serratia marcescens and Filifactor alocis (p < 0.05). In contrast, Fusobacterium necrophorum, Lactobacillus acidophilus, Staphylococcus aureus and Streptococcus pneumoniae were associated with periodontal health (p < 0.05). Pathogenic species of medical importance may be detected in high prevalence and levels in the periodontal microbiota. Regardless of their role in periodontal health or disease, the periodontal biofilm may be a source for dissemination and development of systemic infections by these pathogenic microorganisms. (C) 2015 Elsevier Ltd. All rights reserved.