Three-dimensional cancer cell culture in high-yield multiscale scaffolds by shear spinning
Three-dimensional cancer cell culture in high-yield multiscale scaffolds by shear spinning
复制标题
通过剪切旋转在高产多尺度支架中进行三维癌细胞培养
DOI：
10.1002/btpr.2750
复制
发表时间：
2018
影响因子：
2.9
通讯作者：
Ahmed A
中科院分区：
文献类型：
--
作者：
Ahmed A
由微纤维和亚微米纤维两种尺度组成的聚合物支架可以更好地支持组织工程中三维(3D)细胞的生长，使其成为一类重要的医疗保健材料。然而，一个主要的制造障碍阻碍了它们转化为更广泛的实际应用：可伸缩性。传统的双尺度电纺脚手架生产速度慢，成本高。对于日常细胞培养，支架需要负担得起，以高产量制造，以降低成本。结合英国和美国学术界和工业界的专业知识，这项研究使用了一系列新的高产量、低成本的剪切纺丝制成的组织工程支架。扫描电子显微镜下观察到，支架由相互交织的亚微米纤维和微纤维组成，并显示出良好的支持细胞培养用于肿瘤建模的能力。将3株稳定表达绿色荧光蛋白的模型人癌细胞系(HEK293、A549和MCF7)培养在支架上，发现它们具有高效的细胞贴壁和持续的三维生长和增殖能力，可持续30 天。冰冻切片和多光子荧光显微镜证实支架内形成了致密的3D细胞团。此外，2D和3D培养的比较生长曲线显示出显著的细胞类型依赖性差异。这项工作将高产量剪切纺丝支架应用于哺乳动物组织工程，并使多尺度支架的实际、负担得起的应用更接近现实。2018年美国化学工程师学会生物技术。程序，35：E2750,2019年。
Polymeric scaffolds comprising two size scales of microfibers and submicron fibers can better support three‐dimensional (3D) cell growth in tissue engineering, making them an important class of healthcare material. However, a major manufacturing barrier hampers their translation into wider practical use: scalability. Traditional production of two‐scale scaffolds by electrospinning is slow and costly. For day‐to‐day cell cultures, the scaffolds need to be affordable, made in high yield to drive down cost. Combining expertise from academia and industry from the United Kingdom and United States, this study uses a new series of high‐yield, low‐cost scaffolds made by shear spinning for tissue engineering. The scaffolds comprise interwoven submicron fibers and microfibers throughout as observed under scanning electron microscopy and demonstrate good capability to support cell culturing for tumor modeling. Three model human cancer cell lines (HEK293, A549 and MCF‐7) with stable expression of GFP were cultured in the scaffolds and found to exhibit efficient cell attachment and sustained 3D growth and proliferation for 30 days. Cryosection and multiphoton fluorescence microscopy confirmed the formation of compact 3D cell clusters throughout the scaffolds. In addition, comparative growth curves of 2D and 3D cultures show significant cell‐type‐dependent differences. This work applies high‐yield shear‐spun scaffolds in mammalian tissue engineering and brings practical, affordable applications of multiscale scaffolds closer to reality. © 2018 American Institute of Chemical EngineersBiotechnol. Prog., 35: e2750, 2019.