Evaluation of the diagnostic potential of antibodies to beta2-glycoprotein 1 domain 1 in Chinese patients with antiphospholipid syndrome.
Evaluation of the diagnostic potential of antibodies to beta2-glycoprotein 1 domain 1 in Chinese patients with antiphospholipid syndrome.
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β2-糖蛋白 1 结构域 1 抗体对中国抗磷脂综合征患者的诊断潜力评估
DOI：
10.1038/srep23839
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发表时间：
2016-04-07
影响因子：
4.6
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Zhang S;Wu Z;Chen S;Li J;Wen X;Li L;Zhang W;Zhao J;Zhang F;Li Y
本研究评价抗β2-糖蛋白1结构域1抗体（aβ 2GP 1-D1）诊断抗磷脂综合征（APS）的临床价值。检测了229例受试者的血清，包括35例原发性APS患者、51例与其他疾病相关的APS患者、30例非APS血栓形成患者、32例非APS妊娠相关发病患者、42例系统性红斑狼疮患者和39例健康对照（HC）。用化学发光法测定血清IgG aβ 2GP 1-D1、抗心磷脂（aCL）IgG/IgM和IgG/IgM aβ 2GP 1。APS患者IgG aβ 2GP 1-D1水平显著高于疾病对照组和HC（p < 0.001）。IgG aβ 2GP 1-D1与IgG aβ 2GP 1之间存在显著相关性（p < 0.0001），表明IgG aβ 2GP 1-D1是IgG aβ 2GP 1家族中主要的结构域特异性抗体。重要的是，β 2GP 1-D1与血栓形成事件显著相关，而β 2GP 1非D1与血栓形成事件无关。有趣的是，未观察到IgG aβ 2GP 1-D1与产科并发症之间的显著相关性。此外，aPL三重阳性患者的IgG aβ 2GP 1-D1水平显著高于aPL双重和单一阳性患者。我们的研究结果表明，IgG aβ 2GP 1-D1在识别具有血栓形成高风险的APS患者中具有潜在的作用，从而为在中国引入IgG aβ 2GP 1-D1提供了见解。
In this study, we evaluated the clinical performance of anti-β2-glycoprotein 1 domain 1 antibodies (aβ2GP1-D1) in the diagnosis of antiphospholipid syndrome (APS). Sera from 229 subjects were tested, including 35 patients with primary APS, 51 patients with APS associated to other diseases, 30 patients with non-APS thrombosis, 32 patients with non-APS pregnancy-related morbidity, 42 patients with systemic lupus erythematosus, and 39 healthy controls (HC). Serum IgG aβ2GP1-D1, IgG/IgM anti-cardiolipin (aCL) and IgG/IgM aβ2GP1 were measured by a chemiluminescence assay. The levels of IgG aβ2GP1-D1 were significantly increased in patients with APS, compared with disease controls and HCs (p < 0.001). Significant correlation was identified between IgG aβ2GP1-D1 and IgG aβ2GP1 (p < 0.0001), indicating IgG aβ2GP1-D1 were the predominant domain-specific antibodies in IgG aβ2GP1 family. Importantly, aβ2GP1-D1, but not aβ2GP1 non-D1, was significantly correlated with thrombotic events. Interestingly, no significant correlation between IgG aβ2GP1-D1 and obstetric complications was observed. Additionally, significantly higher levels of IgG aβ2GP1-D1 were found in patients with triple aPL positivity, compared with patients with double and single aPL positivity. Our findings suggest a potential role of IgG aβ2GP1-D1 in identifying APS patients with high risk of thrombosis, shedding insight on the introduction of IgG aβ2GP1-D1 in China.