Effects of short-term heat stress on oxidative damage and responses of antioxidant system in Lilium longiflorum
Effects of short-term heat stress on oxidative damage and responses of antioxidant system in Lilium longiflorum
复制标题
DOI：
10.1007/s10725-007-9227-6
复制
发表时间：
2007-09
影响因子：
4.2
通讯作者：
Hui-fang Yin;Qiuming Chen;M. Yi
中科院分区：
文献类型：
--
作者：
Hui-fang Yin;Qiuming Chen;M. Yi
本文旨在测定百合(Lilium long giflorum L.)抗氧化系统，以适应短期高温。将植物置于37℃、42℃、47℃三种水平的高温胁迫下10h，测定过氧化氢(H_2O_2)和超氧化物歧化酶(O2-−)的产生速率以及膜伤害指数，并测定抗氧化剂的变化。与对照(20℃)相比，37℃和42℃处理10h后，电解质渗漏率和丙二醛浓度变化不大，而47℃处理后显著增加。在37℃和42℃处理10h内，超氧化物歧化酶、过氧化氢酶和谷氨酸脱氢酶等抗氧化物酶活性增强，抗氧化剂(AsA和GSH)浓度维持在较高水平，导致O2-−和H2O2浓度较低。47℃处理10h后，超氧化物歧化酶、过氧化氢酶、GR活性和谷胱甘肽含量与对照相似，而过氧化氢酶、过氧化氢酶活性和抗坏血酸含量显著低于对照，而O2、−和H_2O_2浓度显著升高。此外，超氧化物歧化酶和过氧化物酶同工酶也证实了热诱导对抗氧化酶的影响，因为铜/锌超氧化物歧化酶在热胁迫下保持了较高的稳定性，随着热胁迫时间的延长，特别是在47℃下，过氧化物酶同工酶的强度降低。
This paper aims to determine the changes in reactive oxygen species (ROS) and the responses of the lily (Lilium longiflorumL.) antioxidant system to short-term high temperatures. Plants were exposed to three levels of heat stress (37°C, 42°C, 47°C) for 10 h when hydrogen peroxide (H2O2) and superoxide (O2−) production rate along with membrane injury indexes, and changes in antioxidants were measured. Compared with the control (20°C), electrolyte leakage and MDA concentration varied slightly after 10 h at 37°C and 42°C, while increased significantly at 47°C. During 10 h at 37°C and 42°C, antioxidant enzyme activities, such as SOD, POD, CAT, APX and GR, were stimulated and antioxidants (AsA and GSH concentrations) maintained high levels, which resulted in low levels of O2−and H2O2concentration. However, after 10 h at 47°C, SOD, APX, GR activities and GSH concentration were similar to the controls, while POD, CAT activities and AsA concentration decreased significantly as compared with the control, concomitant with significant increase in O2−and H2O2concentrations. In addition, such heat-induced effects on antioxidant enzymes were also confirmed by SOD and POD isoform, as Cu/ZnSOD maintained high stability under heat stress and the intensity of POD isoforms reduced with the duration of heat stress, especially at 47°C. It is concluded that in lily plants, the oxidative damage induced by heat stress was related to the changes in antioxidant enzyme activities and antioxidants.