INVIVO AND INVITRO MACROPHAGE ACTIVATION BY SYSTEMIC ADJUVANTS
INVIVO AND INVITRO MACROPHAGE ACTIVATION BY SYSTEMIC ADJUVANTS
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DOI：
10.1007/bf01972217
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
MATHE, G
中科院分区：
文献类型：
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作者：
BRULEYROSSET, M;FLORENTIN, I;MATHE, G
测试了六种全身免疫佐剂诱导巨噬细胞活化的能力。其中四种：活卡介苗、卡介苗 (HIU II) 和 M 的水溶性提取物。耻垢分枝杆菌 (IPM) 和来自大肠杆菌的脂多糖。当给正常小鼠施用大肠杆菌（LPS）时，巨噬细胞在体外对肿瘤细胞具有非特异性细胞毒性。这种现象的强度根据佐剂给药的途径和时间而变化。相比之下，从香菇中提取的香菇多糖和合成化合物左旋咪唑可降低巨噬细胞的细胞毒性潜力。BCG、IMP 和 LPS 被证明对巨噬细胞有直接作用。体外暴露于这些药物后，正常巨噬细胞的细胞毒性潜力大大增加。左旋维明索不能在体外直接刺激这种巨噬细胞功能。另一方面，当正常巨噬细胞在来自人淋巴母细胞系上清液的 MIF 存在下培养时，这种巨噬细胞活化已在体外被诱导。
Six systemic adjuvants of immunity were tested for their ability to induce macrophage activation. Four of them: living BCG, hydrosoluble extracts from BCG (HIU II) and fromM. smegmatis(IPM), and lipopolysaccharide fromE. coli(LPS), when administered to normal mice render macrophages non-specifically cytotoxic for tumor cells in vitro. The intensity of this phenomenon varied according to the route and time of adjuvant administration. In contrast, lentinan extracted fromLentinus edodes, and levamisole which is a synthetic chemical compound, depressed macrophage cytotoxic potential.BCG, IMP and LPS were shown to have a direct action on macrophages. After in vitro exposure to these agents, the cytotoxic potential of normal macrophages was greatly increased. Levaminsole was unable ot stimulate this macrophage function directly in vitro. On the other hand, such a macrophage activation has been induced in vitro when normal macrophages were cultivated in the presence of MIF coming from the supernatant of human lymphoblastoid cell lines.