Complete amino acid sequence of the type II isozyme of rat hexokinase, deduced from the cloned cDNA: comparison with a hexokinase from novikoff ascites tumor.
Complete amino acid sequence of the type II isozyme of rat hexokinase, deduced from the cloned cDNA: comparison with a hexokinase from novikoff ascites tumor.
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从克隆的 cDNA 推导出的大鼠己糖激酶 II 型同工酶的完整氨基酸序列：与来自 novikoff 腹水瘤的己糖激酶进行比较。
DOI：
10.1016/0003-9861(91)90094-y
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发表时间：
1991
影响因子：
3.9
通讯作者：
Wilson,JE
中科院分区：
文献类型：
--
作者：
Thelen,AP;Wilson,JE
从克隆的cDNA序列中推导出大鼠己糖激酶II型同工酶的917残基氨基酸序列。测定了5′非翻译区197个核苷酸和3′非翻译区687个碱基的序列。通过分离和测序跨越该区域的基因组DNA克隆，证实了两个离散cDNA克隆之间的重叠区域。在该区域内，634个核苷酸的编码序列被分成3个外显子，每个外显子有150-250个核苷酸；这些结果表明编码II型己糖激酶的基因可能相当复杂。II型同工酶的N端和c端序列之间存在广泛的相似性，正如先前在I型和III型同工酶中看到的那样；这与认为这些酶是通过基因复制和融合过程进化而来的观点是一致的。从Novikoff腹水肿瘤细胞中分离出编码己糖激酶整个c端一半的cDNA，并发现与编码骨骼肌II型同工酶相应区域的cDNA相同。Northern分析表明，一个长度约为5200个核苷酸的mRNA编码了骨骼肌和肿瘤酶。这些结果不支持先前关于正常组织中己糖激酶同工酶与肿瘤组织中己糖激酶同工酶不同的推测，并提示单一蛋白质物种的翻译后修饰可能解释了正常组织和肿瘤组织中同工酶之间的明显差异。
The 917-residue amino acid sequence of the Type II isozyme of rat hexokinase has been deduced from the nucleotide sequence of cloned cDNA. The sequences of 197 nucleotides in the 5′ untranslated region and 687 bases of the 3′ untranslated region have also been determined. A region of overlap between two discrete cDNA clones was confirmed by isolation and sequencing of a genomic DNA clone that spanned the region. Within this region, the 634-nucleotide coding sequence was divided into three exons, each of 150–250 nucleotides; these results suggest that the gene encoding Type II hexokinase is likely to be quite complex. There is extensive similarity between the sequences of the N- and C-terminal halves of the Type II isozyme, as previously seen with the Type I and III isozymes; this is consistent with the view that these enzymes evolved by a process of gene duplication and fusion. A cDNA encoding the entire C-terminal half of a hexokinase from Novikoff ascites tumor cells was also isolated and found to be identical to a cDNA encoding the corresponding region of the Type II isozyme of skeletal muscle. Northern analysis indicated that a single mRNA, approx 5200 nucleotides in length, encoded both the skeletal muscle and the tumor enzymes. These results do not support previous speculation that the hexokinase isozymes of normal tissue are distinct from those of tumors, and suggest the possibility that post-translational modifications of a single protein species might account for apparent differences between the isozymes of normal and tumor tissues.
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大鼠脑己糖激酶：变构调节位点位于酶 N 末端结构域中。
影响因子：
3.9
作者：
White,TK;Wilson,JE
通讯作者：
Wilson,JE
大鼠脑己糖激酶的蛋白水解解剖：胰蛋白酶有限消化过程中切割模式的测定。
影响因子：
3.9
作者：
Polakis,PG;Wilson,JE
通讯作者：
Wilson,JE
人己糖激酶：氨基端和羧基端半部分的序列是同源的。
DOI：
--
发表时间：
1988
期刊：
Biochemical and Biophysical Research Communications - BBRC
影响因子：
--
作者：
S. Nishi;S. Seino;G. Bell
通讯作者：
G. Bell
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影响因子：
2.9
作者：
S. S. Qadri;J. S. Easterby
通讯作者：
J. S. Easterby
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影响因子：
3.9
作者：
T. White;J. Wilson
通讯作者：
J. Wilson