RELATIVE PERMISSIVENESS OF MACROPHAGES FROM BLACK AND WHITE PEOPLE FOR VIRULENT TUBERCLE-BACILLI
RELATIVE PERMISSIVENESS OF MACROPHAGES FROM BLACK AND WHITE PEOPLE FOR VIRULENT TUBERCLE-BACILLI
复制标题
DOI：
10.1128/iai.58.3.632-638.1990
复制
发表时间：
1990-03-01
影响因子：
3.1
通讯作者：
ELKINS, N
中科院分区：
文献类型：
--
作者：
CROWLE, AJ;ELKINS, N
流行病学、临床和组织病理学证据表明，黑人比白色人更易患结核病。通过比较来自黑人和白色供体的血液来源的巨噬细胞对毒性结核杆菌实验感染的反应，在体外研究了这种推定易感性的细胞基础。来自成对的黑色和白色供体的吞噬细胞被感染。通过显微镜计数和0、4和7天时的细菌CFU计数来测量这些细胞中结核杆菌的摄取和复制。还研究了供体血清、1，25-（OH）_2-维生素D_3和γ-干扰素对感染的影响。在吞噬过程中，黑色供体吞噬细胞比白色供体吞噬细胞杀死更多的杆菌。然而，在成功感染的巨噬细胞中，黑色供体的杆菌生长一致且显著快于白色供体，尤其是在存在黑色供体血清的情况下。1，25-（OH）2-维生素D3对来自黑人供体的巨噬细胞的抗结核杆菌保护显著低于来自白色供体的巨噬细胞。两个种族的巨噬细胞的宽容性受到γ干扰素的影响相同。这些结果表明，与白色人相比，黑人的单核细胞吞噬细胞和血清中存在一些固有的和环境的不利因素，这可能有助于他们对结核病的易感性。
Epidemiological, clinical, and histopathological evidence suggests that black people are more susceptible to tuberculosis than are white people. The cellular basis of this putative susceptibility was investigated in vitro by comparing responses of blood-derived macrophages from black and white donors to experimental infection with virulent tubercle bacilli. Phagocytes from pairs of black and white donors were infected. The uptake and replication of the tubercle bacilli in these cells were measured by microscopic counts and by CFU counts of bacilli at 0, 4, and 7 days. The effects of donor serum, of 1,25-(OH)2-vitamin D3, and gamma interferon on the infection also were studied. Black-donor phagocytes killed more bacilli during phagocytosis than white-donor phagocytes did. However, the bacilli grew consistently and significantly faster in successfully infected macrophages from black than from white donors, especially in the presence of black-donor serum. 1,25-(OH)2-vitamin D3 gave significantly less protection against tubercle bacilli to macrophages from black donors than macrophages from white donors. The permissiveness of the macrophages from the two races were affected equally by gamma interferon. These results demonstrate some inherent and environmental liabilities in the monocytic phagocytes and serum of black people compared with white people, which may contribute to their greater susceptibility to tuberculosis.