TRACTION FORCES IN LOCOMOTING CELLS
TRACTION FORCES IN LOCOMOTING CELLS
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DOI：
10.1002/cm.970310306
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发表时间：
1995-01-01
影响因子：
--
通讯作者：
JACOBSON, K
中科院分区：
文献类型：
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作者：
OLIVER, T;DEMBO, M;JACOBSON, K
已经开发出一种确定运动细胞对基质施加的牵引力的定量图的方法。该方法类似于 Harris 硅胶基质测定 [Harris 等人，1980：Science 208.177-179]，但使用改进的无皱薄膜，该薄膜在响应牵引力时变形更可预测。该方法还利用橡胶变形的数学分析来生成牵引力分布的最终图。由此产生的图一致表明，鱼类角膜细胞在基质上施加稳态“夹紧”，垂直于细胞的运动方向。在单元的前边缘处或附近没有检测到明显的向后牵引力。同样，在细胞背面没有发现与粘连剥离相关的显着向前牵引力。第二种测定使用轻轻附着的玻璃微针的偏转来测量运动细胞施加的总力。需要大约 4.5 x 10(-3) dyn 的力才能“停止”运动的角膜细胞。讨论了这些发现对细胞运动的影响。 (C) 1995 Wiley-Liss, Inc.
A means of determining quantitative maps of the tractions exerted by locomoting cells on a substratum has been developed. This method is similar to the Harris silicone substratum assay [Harris et al., 1980: Science 208.177-179], but uses an improved non-wrinkling film that deforms more predictably in response to traction forces. The method also utilizes a mathematical analysis of rubber deformation to produce the final map of the distribution of tractions. The resulting maps consistently showed that fish keratocytes exert a steady-state ''pinching'' on the substratum, perpendicular to the cell's direction of locomotion. No significant rearward tractions were detected at or near the front edge of the cell. Likewise, no significant forward tractions associated with peeling of adhesions were found at the back of the cell. A second assay uses deflection of a lightly attached glass microneedle to measure the total force exerted by locomoting cells. Forces of approximately 4.5 x 10(-3) dyn were required to ''stall'' locomoting keratocytes. The implications of these findings for cell movement are discussed. (C) 1995 Wiley-Liss, Inc.