EGF-R and erbB-2 in murine tooth development after ethanol exposure
EGF-R and erbB-2 in murine tooth development after ethanol exposure
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DOI：
10.1002/bdra.20113
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发表时间：
2005-02-01
影响因子：
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通讯作者：
Hernández-Guerrero, JC
中科院分区：
文献类型：
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作者：
Jiménez-Farfán, D;Guevara, J;Hernández-Guerrero, JC
背景：怀孕期间饮酒经常会导致一种称为胎儿酒精综合征(Fas)的先天性疾病；然而，并不是所有酗酒妇女所生的孩子都会患上Fas。饮酒可能会影响胚胎发育期间的各种器官和系统，包括头面部结构。酒精暴露后，可以观察到牙齿变小、釉质改变和延迟萌出。表皮生长因子受体(EGF-Rs)参与牙齿的增殖和分化，这些受体的变化被认为是该综合征中观察到的牙齿异常的可能机制。方法：将孕鼠随机分为5、10、15、20、25%乙醇溶液组(对照组)，分别饮用5%、10%、15%、20%、25%乙醇溶液或水。头部取自16.5天和18.5天的胎儿。采用免疫组织化学方法检测EGF-R和erbB-2的表达。结果：在16.5天和18.5天，15%、20%和25%酒精组的胎儿出现分化延迟，牙齿上皮组织退行性改变，牙齿变小，EGF-R和erbB-2免疫反应增强。结论：孕期饮酒影响EGF受体的表达，并导致小鼠胚胎牙齿形态发生延迟。牙齿发育是一个涉及许多生长因子的过程；因此，我们认为需要进一步的研究来表明在其他细胞中观察到的糖基化和生长因子信号通路的变化是否也参与了本研究中观察到的变化。出生缺陷研究(A部分)73：65-71,2005。(C)2005年Wiley-Liss，Inc.
BACKGROUND: Alcohol consumption during pregnancy can frequently lead to a congenital disorder known as fetal alcohol syndrome (FAS); however, not all children born to alcoholic women develop FAS. Alcohol consumption may affect diverse organs and systems during embryonic development, including craniofacial structures. Small teeth, enamel alterations, and delayed eruption have been observed after ethanol exposure. Epidermal growth factor receptors (EGF-Rs) participate in dental proliferation and differentiation, and changes in these receptors were considered here to be a likely mechanism associated to the dental anomalies observed in this syndrome. Epidermal growth factor receptor type 1 (EGF-R) and epidermal growth factor receptor type 2 (erbB-2) immunoexpression during the lower first molar morphogenesis was investigated in mouse fetuses exposed to ethanol during gestation.METHODS: Pregnant female mice were divided into groups, consuming either 5, 10, 15, 20, or 25% ethanol solutions, or water (control group). Heads were obtained from 16.5- and 18.5-day fetuses. Immunohistochemistry was applied to EGF-R and erbB-2. RESULTS: At days 16.5 and 18.5, fetuses from 15%,20%, and 25% ethanol groups showed delayed differentiation, degenerative changes in dental epithelial tissues and reduced dental size; additionally, they displayed an enhanced immunoreactivity to EGF-R and erbB-2. CONCLUSIONS: Our results suggest that ethanol consumption during pregnancy affects the expression of EGF receptors and induces a delay in murine fetal dental morphogenesis. Dental development is a process that involves a number of growth factors; hence we consider that further research is required to show whether the changes in glycosylation and growth-factor signaling pathways observed in other cells are also involved in the alterations observed in this study. Birth Defects Research (Part A) 73: 65-71, 2005. (C) 2005 Wiley-Liss, Inc.