Long-Term Two-Photon Imaging in Awake Macaque Monkey
Long-Term Two-Photon Imaging in Awake Macaque Monkey
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DOI：
10.1016/j.neuron.2017.01.027
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发表时间：
2017-03-08
期刊：
影响因子：
16.2
通讯作者：
Tang, Shiming
中科院分区：
文献类型：
--
作者：
Li, Ming;Liu, Fang;Tang, Shiming
在清醒的猕猴中成功应用双光子成像和遗传工具，将使我们在分子和神经元回路水平上对更高认知功能的理解取得根本性进展。在这里，我们报告技术的长期双光子成像在清醒的猕猴。使用基因编码的指标，包括GCaMP 5和GCaMP 6s由AAV 2/1传递到视觉皮层，我们证明了大的神经元群体的高质量双光子成像可以实现并保持在清醒的猴子数月。同时的细胞内记录和双光子钙成像证实，荧光活性是线性成比例的神经元尖峰活动在很宽的范围内的发射率（10 Hz至150 Hz）。通过在清醒的猴子中以单细胞或单树突棘分辨率提供对皮质神经元群体的双光子成像，报告的技术可以帮助弥合现代遗传和分子工具的使用以及更高认知功能的研究。
Successful application of two-photon imaging with genetic tools in awake macaque monkeys will enable fundamental advances in our understanding of higher cognitive function at the level of molecular and neuronal circuits. Here we report techniques for long-term two-photon imaging in awake macaque monkeys. Using genetically encoded indicators including GCaMP5 and GCaMP6s delivered by AAV2/1 into the visual cortex, we demonstrate that high-quality two-photon imaging of large neuronal populations can be achieved and maintained in awake monkeys for months. Simultaneous intracellular recording and two-photon calcium imaging confirm that fluorescence activity is linearly proportional to neuronal spiking activity across a wide range of firing rates (10 Hz to 150 Hz). By providing two-photon imaging access to cortical neuronal populations at single-cell or single dendritic spine resolution in awake monkeys, the techniques reported can help bridge the use of modern genetic and molecular tools and the study of higher cognitive function.