Inhibition of fatty acid oxygenases by onion and garlic oils. Evidence for the mechanism by which these oils inhibit platelet aggregation.
Inhibition of fatty acid oxygenases by onion and garlic oils. Evidence for the mechanism by which these oils inhibit platelet aggregation.
复制标题
洋葱和大蒜油抑制脂肪酸加氧酶。
DOI：
10.1016/0006-2952(80)90581-x
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发表时间：
1980
影响因子：
5.8
通讯作者：
J. Bailey
中科院分区：
文献类型：
--
作者：
J. Vanderhoek;A. Makheja;J. Bailey
洋葱油和大蒜油抑制体外血小板聚集的同时，[1-14 C]花生四烯酸（AA）生成的血栓烷B 2（TXB 2）和12-羟基十七碳三烯酸（HHT）减少。在中等浓度（10-30 μg/ml）下，这些油还诱导脂氧合酶途径产物的重新分布;在较高浓度（30-60 μg/ml）下，它们完全抑制所有加氧酶产物的形成。抗霉素处理的血小板耗氧量的测量表明，血小板脂肪酸加氧酶的抑制和这些油的抗聚集活性之间的直接相关性。洋葱油和大蒜油也能抑制绵羊泡状腺制备物中的脂肪酸加氧酶，表现为耗氧量降低和[1-14 C] AA生成前列腺素E2（PGE 2）和PGD 2减少。洋葱油在抑制血小板加氧酶方面比来自泡状腺的加氧酶有效约10倍。此外，这些油抑制大豆脂肪氧合酶催化氧化AA。在所有三种酶系统中，洋葱油比大蒜油表现出更大的抑制活性。许多非甾体抗炎和抗血栓形成药物通过抑制前列腺素和血栓烷合成酶的环氧合酶组分起作用。因此，我们的研究结果表明，洋葱和大蒜含有可能具有偶然药理作用的化合物。
Inhibition of in vitro platelet aggregation by onion and garlic oils was accompanied by decreased formation of thromboxane B 2 (TXB 2) and 12-hydroxyheptadecatrienoic acid (HHT) from [1-14 C] arachidonic acid (AA). At intermediate concentrations (10–30 μg ml), these oils also induced a redistribution of the products of the lipoxygenase pathway; at higher concentrations (30–60 μg ml), they completely suppressed the formation of all oxygenase products. Measurements of oxygen consumption in antimycin-treated platelets indicated a direct correlation between inhibition of platelet fatty acid oxygenases and the anti-aggregating activity of these oils. Onion and garlic oils also inhibited fatty acid oxygenases from sheep vesicular gland preparations as shown both by decreased oxygen consumption and decreased formation of prostaglandin E 2 (PGE 2) and PGD 2 from [1-14 C] AA. Onion oil was approximately ten times more effective in inhibiting the platelet oxygenases than the oxygenases from the vesicular gland. In addition, these oils suppressed the soybean lipoxygenase-catalyzed oxygenation of AA. In all three enzyme systems, onion oil exhibited greater inhibitory activity than garlic oil. Many nonsteroidal anti-inflammatory and anti-thrombotic drugs act by inhibiting the cyclooxygenase component of the prostaglandin and thromboxane synthetases. Our findings thus suggest that onion and garlic contain compounds that may have serendipitous pharmacological effects.