Affordable Caenorhabditis elegans tracking system for classroom use.
Affordable Caenorhabditis elegans tracking system for classroom use.
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DOI：
10.17912/micropub.biology.000377
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发表时间：
2021-03-17
影响因子：
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通讯作者：
Vidal-Gadea AG
中科院分区：
文献类型：
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作者：
Leonard N;Vidal-Gadea AG
几十年来，线虫C.秀丽隐杆线虫由于其无与伦比的实验适应性而成为一种杰出的研究生物。这一优势也使这种微小的蠕虫成为高等教育机构科学教学的有吸引力的工具。然而，与这些动物的自动行为评估相关的高昂成本仍然是阻碍它们在本科和高中环境中完全采用的障碍。为了改善这种情况，我们开发了一个廉价的蠕虫跟踪系统，供高中实习生和本科生使用。在过去的两年中，这种跟踪器已成功地用于我们的介绍细胞和分子实验室（BSC 220）在伊利诺伊州州立大学的本科生。在这里，我们描述和演示我们的廉价蠕虫跟踪系统的使用。
For decades the nematode C. elegans has served as an outstanding research organism owing to its unsurpassed experimental amenability. This advantage has also made this tiny worm an attractive vehicle for science instruction across higher learning institutions. However, the prohibitive cost associated with the automated behavioral assessment of these animals remains an obstacle preventing their full adoption in undergraduate and high school settings. To improve this situation, we developed an inexpensive worm tracking system for use by high school interns and undergraduate students. Over the past two years this tracker has been successfully used by undergraduate students in our introductory Cell and Molecular lab (BSC220) at Illinois State University. Here we describe and demonstrate the use of our inexpensive worm tracking system.