"Signal-On" Photoelectrochemical Biosensor for Sensitive Detection of Human T-Cell Lymphotropic Virus Type II DNA: Dual Signal Amplification Strategy Integrating Enzymatic Amplification with Terminal Deoxynucleotidyl Transferase-Mediated Extension
"Signal-On" Photoelectrochemical Biosensor for Sensitive Detection of Human T-Cell Lymphotropic Virus Type II DNA: Dual Signal Amplification Strategy Integrating Enzymatic Amplification with Terminal Deoxynucleotidyl Transferase-Mediated Extension
复制标题
用于灵敏检测人 T 细胞嗜淋巴细胞病毒 II 型 DNA 的“信号开启”光电化学生物传感器：将酶扩增与末端脱氧核苷酸转移酶介导的延伸相结合的双信号放大策略
DOI：
10.1021/acs.analchem.5b00679
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发表时间：
2015
影响因子：
7.4
通讯作者：
Zhu Jun-Jie
中科院分区：
文献类型：
--
作者：
Shen Qingming;Han Li;Fan Gaochao;Zhang Jian-Rong;Jiang Liping;Zhu Jun-Jie
基于酶扩增与末端脱氧核苷酸转移酶 (TdT) 介导的延伸策略，开发了一种新型“信号开启”光电化学 (PEC) 生物传感器，用于灵敏检测人 T 细胞嗜淋巴细胞病毒 II 型 (HTLV-II) DNA。通过双信号放大，光电流信号的强度与 HTLV-II DNA 目标 DNA (tDNA) 的浓度成正比。在该方案中，GR-CdS:Mn/ZnS纳米复合材料被用作光电转换材料，而pDNA被用作tDNA识别单元。此外，TdT介导的延伸和酶信号放大技术被用来提高检测的灵敏度。利用这种新颖的双信号放大策略，PEC DNA 传感器原型可以检测低至 ∼0.033 fM 的 HTLV-II DNA，线性范围为 0.1–5000 fM，即使对于单碱基错配也具有出色的区分能力。这种 PEC DNA 检测开辟了一个有前景的平台，可以检测超低水平的各种 DNA 靶标，从而实现不同疾病的早期诊断。
A novel “signal-on” photoelectrochemical (PEC) biosensor for sensitive detection of human T-cell lymphotropic virus type II (HTLV-II) DNA was developed on the basis of enzymatic amplification coupled with terminal deoxynucleotidyl transferase (TdT)-mediated extension strategy. The intensity of the photocurrent signal was proportional to the concentration of the HTLV-II DNA-target DNA (tDNA) by dual signal amplification. In this protocol, GR-CdS:Mn/ZnS nanocomposites were used as photoelectric conversion material, while pDNA was used as the tDNA recognizing unit. Moreover, the TdT-mediated extension and the enzymatic signal amplification technique were used to enhance the sensitivity of detection. Using this novel dual signal amplification strategy, the prototype of PEC DNA sensor can detect as low as ∼0.033 fM of HTLV-II DNA with a linear range of 0.1–5000 fM, with excellent differentiation ability even for single-base mismatches. This PEC DNA assay opens a promising platform to detect various DNA targets at ultralow levels for early diagnoses of different diseases.